CAJ | 학술논문

背景：在体外和体内关于细胞对于不同的机械牵张反应的大量研究表明，牵张能够刺激成骨。然而鲜有文献报道不同的牵张方式对于同种细胞的影响有何不同。 　　目的：比较不同机械牵张方式对大鼠骨髓间充质干细胞的影响。 　　方法：分离培养大鼠骨髓间充质干细胞，应用自行研制的牵张装置对骨髓间充质干细胞分别施加动态、静态和模拟临床的混合牵张牵张刺激，分别检测3种刺激方式下骨髓间充质干细胞的增殖能力、碱性磷酸酶活性及Runx2基因的mRNA表达，并测量细胞骨钙素的分泌情况。 　　结果与结论：静态牵张组与对照组相比，细胞增殖能力提高18.67%，碱性磷酸酶活性、Runx2表达及骨钙素分泌无明显差异；动态牵张组相对于对照组，细胞碱性磷酸酶活性提高60.33%， Runx2表达上升49.67%，细胞外骨钙素的分泌提高了48%，然而细胞增殖则受到了抑制；混合牵张组相对于对照组，细胞增殖能力稍有上升但无统计学差异，其对碱性磷酸酶活性、Runx2表达以及骨钙素的分泌有一定的促进作用，但没有动态牵张组明显。结果提示，静态牵张能够显著刺激骨髓间充质干细胞的增殖，而动态牵张对于刺激骨髓间充质干细胞成骨向分化作用更为明显，混合牵张方式对于细胞增殖及成骨分化均有一定的促进作用。
배경：재체외화체내관우세포대우불동적궤계견장반응적대량연구표명，견장능구자격성골。연이선유문헌보도불동적견장방식대우동충세포적영향유하불동。 　　목적：비교불동궤계견장방식대대서골수간충질간세포적영향。 　　방법：분리배양대서골수간충질간세포，응용자행연제적견장장치대골수간충질간세포분별시가동태、정태화모의림상적혼합견장견장자격，분별검측3충자격방식하골수간충질간세포적증식능력、감성린산매활성급Runx2기인적mRNA표체，병측량세포골개소적분비정황。 　　결과여결론：정태견장조여대조조상비，세포증식능력제고18.67%，감성린산매활성、Runx2표체급골개소분비무명현차이；동태견장조상대우대조조，세포감성린산매활성제고60.33%， Runx2표체상승49.67%，세포외골개소적분비제고료48%，연이세포증식칙수도료억제；혼합견장조상대우대조조，세포증식능력초유상승단무통계학차이，기대감성린산매활성、Runx2표체이급골개소적분비유일정적촉진작용，단몰유동태견장조명현。결과제시，정태견장능구현저자격골수간충질간세포적증식，이동태견장대우자격골수간충질간세포성골향분화작용경위명현，혼합견장방식대우세포증식급성골분화균유일정적촉진작용。
BACKGROUND:In vitro and in vivo studies of cel response to a variety of mechanical loadings have demonstrated the stimulation of bone formation by loads. However, the effects of different mechanical strains on the same cel s have never been adequately studied by far. OBJECTIVE:To investigate the effects of different mechanical strains on rat bone marrow mesenchymal stem cel s. METHODS:Rat bone marrow mesenchymal stem cel s were isolated and cultured in vitro. Bone marrow mesenchymal stem cel s were subjected to different stimulations including dynamic stretch, static stretch and hybrid stretch through the use of custom-made mechanical stretch device. Cel ular proliferation, alkaline phosphatase activity and mRNA expression of Runx2 of bone marrow mesenchymal stem cel s were detected and the secretion of osteocalcin was evaluated under three different stretch modes respectively. RESULTS AND CONCLUSION:Compared to the control group, cel proliferation increased by 18.67%, however, alkaline phosphatase activity, Runx2 expression and osteocalcin secretion were not changed obviously in the static stretchgroup. Compared to the control group, alkaline phosphatase activity, Runx2 expression and osteocalcin secretion increased by 60.33%, 49.67%and 48%respectively;however, cel proliferation was inhibited, in the dynamic stretch group. Compared to the control group, cel proliferation was slightly, but not significantly, increased in the hybrid stretch group, and the alkaline phosphatase activity, Runx2 expression and osteocalcin secretion increased although the increases were not as apparent as those in the dynamic stretch group. These findings suggest that static mechanical strain can significantly promote cel proliferation, the dynamic mechanical strain more greatly promotes osteogenic differentiation of bone marrow mesenchymal stem cel s, and the hybrid mechanical strain promotes the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cel s.