CAJ | 학술논문

背景：力学信号与骨骼系统的生长发育、修复重建和疾病发生发展有密切联系，其对骨髓间充质干细胞的影响和作用机制值得关注。 　　目的：探讨静水压力刺激对骨髓间充质干细胞分化的影响及相关机制。 　　方法：①短期实验：将人骨髓间充质干细胞分别接种于正常 DMEM 培养基、成骨诱导培养基或含细胞外信号调节激酶1/2抑制剂U0126的DMEM培养基中，利用自制压力加载系统对其施加0，40，80 kPa的静水压强1，4 h。②长期实验：将人骨髓间充质干细胞分别接种于正常 DMEM培养基或成骨诱导培养基，施加40 kPa的静水压强4 h/d，持续14 d，以不施加静水压的细胞作对照。 　　结果与结论：实时定量反转录 PCR 结果显示，经成骨诱导或40 kPa静水压刺激4h后，骨髓间充质干细胞内核心结合因子α1、骨钙素mRNA表达增加，过氧化物酶体增殖物活化受体γ2和脂肪酶mRNA表达降低，80 kPa静水压刺激没有出现这一规律；40 kPa静水压的成骨诱导作用可被U0126部分拮抗。组织化学染色显示40 kPa静水压刺激7 d，骨髓间充质干细胞碱性磷酸酶表达和活性均增加；持续刺激14 d，过氧化物酶体增殖物活化受体γ2和脂肪酶mRNA表达增加。说明一定强度和作用时间的静水压刺激可调节骨髓间充质干细胞分化，其作用部分依赖于细胞外信号调节激酶1/2信号途径。
배경：역학신호여골격계통적생장발육、수복중건화질병발생발전유밀절련계，기대골수간충질간세포적영향화작용궤제치득관주。 　　목적：탐토정수압력자격대골수간충질간세포분화적영향급상관궤제。 　　방법：①단기실험：장인골수간충질간세포분별접충우정상 DMEM 배양기、성골유도배양기혹함세포외신호조절격매1/2억제제U0126적DMEM배양기중，이용자제압력가재계통대기시가0，40，80 kPa적정수압강1，4 h。②장기실험：장인골수간충질간세포분별접충우정상 DMEM배양기혹성골유도배양기，시가40 kPa적정수압강4 h/d，지속14 d，이불시가정수압적세포작대조。 　　결과여결론：실시정량반전록 PCR 결과현시，경성골유도혹40 kPa정수압자격4h후，골수간충질간세포내핵심결합인자α1、골개소mRNA표체증가，과양화물매체증식물활화수체γ2화지방매mRNA표체강저，80 kPa정수압자격몰유출현저일규률；40 kPa정수압적성골유도작용가피U0126부분길항。조직화학염색현시40 kPa정수압자격7 d，골수간충질간세포감성린산매표체화활성균증가；지속자격14 d，과양화물매체증식물활화수체γ2화지방매mRNA표체증가。설명일정강도화작용시간적정수압자격가조절골수간충질간세포분화，기작용부분의뢰우세포외신호조절격매1/2신호도경。
BACKGROUND:Mechanical signal has close correlation with the growth, development, repair and reconstruction of the skeletal system and the development of disease, the effect and the mechanism on bone marrow mesenchymal stem cel s is worthy to concern. OBJECTIVE:To explore the effect and mechanism of hydrostatic pressures on the differentiation of bone marrow mesenchymal stem cel s. METHODS:Short-term experiment:the human bone marrow mesenchymal stem cel s were incubated into the normal Dulbecco’s modified Eagle’s medium, osteogenic medium or the Dulbecco’s modified Eagle’s medium containing extracel ular signal-regulated kinase 1/2 inhibitor U0126, respectively. Homemade pressure loading system was used to impose 0, 40 and 80 kPa hydrostatic pressure for 1 and 4 hours. Long-term experiment:human bone marrow mesenchymal stem cel s were incubated into the normal Dulbecco’s modified Eagle’s medium or osteogenic medium respectively, and then 40 kPa hydrostatic pressures was loaded for 4 hours per day, and lasted for 14 days. The cel s without hydrostatic pressure were regarded as the control group. RESULTS AND CONCLUSION:Real-time quantitative reverse transcription PCR results showed that after osteogenic induction and simulated with 40 kPa hydrostatic pressure for 4 hours, the mRNA expressions of core binding factorα1 and osteocalcin in the bone marrow mesenchymal stem cel s were increased, while the mRNA expressions of peroxisome proliferator-activated receptorγ2 and adipsin were decreased, and the 80 kPa hydrostatic pressure did not cause such reactivity. The osteogenic induction effect of 40 kPa hydrostatic pressure could be partial antagonized with U0126. Histochemical staining showed that after simulated with 40 kPa hydrostatic pressure for 7 days, the expression and activity of alkaline phosphatase of bone marrow mesenchymal stem cel s were increased;after lasted for 14 days, the mRNA expressions of peroxisome proliferator-activated receptorγ2 and adipsin were increased. Certain intensity and duration of hydrostatic pressure stimulation can regulate the differentiation of bone marrow mesenchymal stem cel s, and the mechanism is only partly mediated by the extracel ular signal-regulated kinase 1/2 signaling pathway.