CAJ | 학술논문

背景：前期研究发现MCM3与氟中毒相关，但其在氟中毒早期暴露者中的表达情况尚不清楚。 　　目的：分析氟暴露患者及对照人群外周血中MCM3 mRNA的表达量。 　　方法：选取饮水型氟中毒轻度患者(暴露组)、对照人群(非暴露组)各11例，采用SYBRGreen1嵌合荧光法实时定量PCR检测外周血单个核细胞中MCM3 mRNA的表达，同时测定两组肝肾功能指标。 　　结果与结论：暴露组及非暴露组MCM3 mRNA表达量分别为0.57360±0.10259、0.5500±0.17181，两组差异无显著性意义(P>0.05)。所测的肝肾功能指标在两组中差异无显著性意义。结果显示轻度氟暴露对患者外周血单个核细胞中MCM3 mRNA表达无显著影响。氟对暴露者肝、肾功能的影响需要选取更多的指标综合分析。
배경：전기연구발현MCM3여불중독상관，단기재불중독조기폭로자중적표체정황상불청초。 　　목적：분석불폭로환자급대조인군외주혈중MCM3 mRNA적표체량。 　　방법：선취음수형불중독경도환자(폭로조)、대조인군(비폭로조)각11례，채용SYBRGreen1감합형광법실시정량PCR검측외주혈단개핵세포중MCM3 mRNA적표체，동시측정량조간신공능지표。 　　결과여결론：폭로조급비폭로조MCM3 mRNA표체량분별위0.57360±0.10259、0.5500±0.17181，량조차이무현저성의의(P>0.05)。소측적간신공능지표재량조중차이무현저성의의。결과현시경도불폭로대환자외주혈단개핵세포중MCM3 mRNA표체무현저영향。불대폭로자간、신공능적영향수요선취경다적지표종합분석。
BACKGROUND:Previous studies have shown that minichromosome maintenance 3 is related with fluorosis, but the expression of minichromosome maintenance 3 in fluorosis patients is not clear yet. OBJECTIVE:To analyze the mRNA expression level of minichromosome maintenance 3 in peripheral blood from patients exposed to fluoride and normal controls. METHODS:Eleven patients with mild fluorosis by drinking water (exposure group) and 11 cases of control (non-exposure group) were selected for research. SYBRGreen1 real-time quantitative PCR was used to determine the mRNA expression of minichromosome maintenance 3 in peripheral blood mononuclear cel s, and the liver and renal function indicators were detected. RESULTS AND CONCLUSION:mRNA expressions of minichromosome maintenance 3 in the exposure group and non-exposure group were (0.573 60±0.102 59) and (0.550 0±0.171 81), respectively, and there was no significant difference between two groups (P>0.05). There were no significant differences in the liver and renal function indicators between two groups. The results indicate that mild fluorosis has no significant effect on mRNA expression of minichromosome maintenance 3 in the peripheral blood mononuclear cel s. More indicators are needed to compressively analyze the effect of fluoride on the liver and renal functions.