CAJ | 학술논문

背景：良好的股骨头坏死动物模型的建立，有助于研究股骨头坏死的发病机制，为股骨头坏死的预防和治疗提供理论依据。 　　目的：研究内毒素脂多糖联合地塞米松注射液诱导兔股骨头坏死的实验效果。 　　方法：新西兰大白兔36只随机分为模型组21只和对照组15只。模型组连续2 d每日经耳缘静脉注射内毒素脂多糖10μg/kg，再连续3 d，每日肌肉注射地塞米松注射液25 mg/kg；对照组注射同等剂量的生理盐水。 　　结果与结论：模型组4周后X射线显示兔关节间隙增宽，密度增大，关节软骨下骨密度增高，股骨头变平，骨小梁模糊，软骨下骨与骨松质界限不清，在股骨头内出现斑块状高密度区域，股骨颈变短粗。双能量X射线骨密度测量仪进行股骨头局部骨密度测量，兔股骨头骨密度检测发现模型组股骨头骨密度、骨矿物质含量均显著低于对照组(P<0.05)。组织学切片见模型组骨细胞陷窝空疏，脂肪细胞增多，部分血管栓塞，其中存活动物的骨坏死率和骨陷窝率均明显高于对照组。证实地塞米松联合脂多糖可有效建立激素性股骨头坏死模型。
배경：량호적고골두배사동물모형적건립，유조우연구고골두배사적발병궤제，위고골두배사적예방화치료제공이론의거。 　　목적：연구내독소지다당연합지새미송주사액유도토고골두배사적실험효과。 　　방법：신서란대백토36지수궤분위모형조21지화대조조15지。모형조련속2 d매일경이연정맥주사내독소지다당10μg/kg，재련속3 d，매일기육주사지새미송주사액25 mg/kg；대조조주사동등제량적생리염수。 　　결과여결론：모형조4주후X사선현시토관절간극증관，밀도증대，관절연골하골밀도증고，고골두변평，골소량모호，연골하골여골송질계한불청，재고골두내출현반괴상고밀도구역，고골경변단조。쌍능량X사선골밀도측량의진행고골두국부골밀도측량，토고골두골밀도검측발현모형조고골두골밀도、골광물질함량균현저저우대조조(P<0.05)。조직학절편견모형조골세포함와공소，지방세포증다，부분혈관전새，기중존활동물적골배사솔화골함와솔균명현고우대조조。증실지새미송연합지다당가유효건립격소성고골두배사모형。
BACKGROUND:To construct a normal animal model of femoral head necrosis contributes to the research of the pathogenesis of femoral head necrosis, which can provide theoretical basis for the prevention and treatment of femoral head necrosis. OBJECTIVE:To research the experimental effect of lipopolysaccharide combined with dexamethasone injection in the induction of rabbit femoral head necrosis. METHODS:Thirty-six New Zealand white rabbits were randomly divided into model group (n=21) and control group (n=15). The rabbits in the model group were injected with 10μg/kg lipopolysaccharide daily and continuous for 2 days, and then injected with 25 mg/kg dexamethasone daily for 3 days continuously. The rabbits in the control group were injected with the normal saline at the same volume. RESULTS AND CONCLUSION:After 4 weeks, the X-ray film of the rabbit in the model group showed the joint gaps were widened, the density was increased, the articular subchondral bone mineral density was increased, the femoral head was flat, trabecular bone was fuzzy, the boundaries between subchondral bone and cancel ous bone was unclear, and the patchy high-density areas were observed in the femoral head with shortened femoral neck. The bone mineral density of partial femoral head was measured with dual-energy X-ray absorptiometry, and found that the bone mineral density of femoral head and the bone mineral content of the model group were significantly lower than those of the control group (P<0.05). Histological section observation showed that the bone cel lacuna was empty and shal ow, fat cel s were increased and vascular thrombosis was observed, meanwhile, the osteonecrosis rate and lacunae rate of the survival animals were significantly higher than those in the control group. Dexamethasone combined with lipopolysaccharide can effectively construct the model of steroid-induced avascular necrosis of the femoral head.