CAJ | 학술논문

背景：病理情况下，细胞间黏附因子1表达明显升高，促进炎症反应的发生，加重脑组织损伤。 　　目的：观察眼针穴区、穴区外治疗对急性脑缺血再灌注损伤模型大鼠海马组织细胞间黏附因子1表达的影响。 　　方法：40只SD大鼠随机等分为正常组、假手术组、模型组、眼针穴区组和穴区外组。后3组用线栓法复制脑缺血再灌注损伤大鼠模型，栓塞线插入深度1.8-2.2 cm，眼针组于脑缺血再灌注即刻及取材前30 min，取肝区、上焦区、下焦区、肾区平刺眼眶周治疗20 min。穴区外组选择眼针同名穴区的外3 mm处为针刺部位，刺法与眼针穴区相同。 　　结果与结论：眼针穴区治疗后大鼠神经功能缺损评分下降，海马组织细胞间黏附因子1蛋白及mRNA表达显著降低(P <0.01)。穴区外组治疗后大鼠神经功能缺损评分及海马组织细胞间黏附因子1蛋白及mRNA表达无明显变化。提示眼针具有明显的改善大鼠脑缺血再灌注损伤的作用，其机制与下调海马组织细胞间黏附因子表达有关。
배경：병리정황하，세포간점부인자1표체명현승고，촉진염증반응적발생，가중뇌조직손상。 　　목적：관찰안침혈구、혈구외치료대급성뇌결혈재관주손상모형대서해마조직세포간점부인자1표체적영향。 　　방법：40지SD대서수궤등분위정상조、가수술조、모형조、안침혈구조화혈구외조。후3조용선전법복제뇌결혈재관주손상대서모형，전새선삽입심도1.8-2.2 cm，안침조우뇌결혈재관주즉각급취재전30 min，취간구、상초구、하초구、신구평자안광주치료20 min。혈구외조선택안침동명혈구적외3 mm처위침자부위，자법여안침혈구상동。 　　결과여결론：안침혈구치료후대서신경공능결손평분하강，해마조직세포간점부인자1단백급mRNA표체현저강저(P <0.01)。혈구외조치료후대서신경공능결손평분급해마조직세포간점부인자1단백급mRNA표체무명현변화。제시안침구유명현적개선대서뇌결혈재관주손상적작용，기궤제여하조해마조직세포간점부인자표체유관。
BACKGROUND:Significant increasing of intercel ular adhesion molecule 1 expression can promote the occurrence of inflammatory response and increase brain tissue injury. OBJECTIVE:To investigate the effect of eye acupuncture point and non-point therapy on intercel ular adhesion molecule 1 expression in rat hippocampus of acute cerebral ischemia-reperfusion injury model. METHODS:Forty Sprague Dawley rats were divided into normal group, sham-operation group, model group, eye acupuncture point group and eye acupuncture non-point group. Rats in the model group, eye acupuncture point group and eye acupuncture non-point group were used to establish the acute cerebral ischemia-reperfusion injury model with suture method, the suture was deep for 1.8-2.2 cm. In the eye acupuncture point group, the acupuncture points of hepatic region, upper energizer area, lower energizer area and kidney area were selected when cerebral ischemia-reperfusion occurred immediately and 30 minutes before drawing materials, then horizontal needling the acupuncture points around the orbit for 20 minutes. In the eye acupuncture non-point group, the puncture site was selected at 3 mm away from eye acupuncture non-point area, and then needled the puncture site with the same method in the eye acupuncture point group. RESULTS AND CONCLUSION:After eye acupuncture point therapy, the neurological deficit scores were decreased, and the intercel ular adhesion molecule 1 expression in rat hippocampus was significantly decreased (P<0.01). After eye acupuncture non-point therapy, there were no significant changes in neurological deficit scores and the expression of intercel ular adhesion molecule 1 protein and mRNA in rat hippocampus. The results indicate that eye acupuncture can significantly improve the rat cerebral ischemia-reperfusion injury, and the mechanism may relate with reducing the intercel ular adhesion molecule 1 expression in rat hippocampus.