中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
37期
6587-6593
,共7页
闫鹏%郑伟东%张季凯%谭云冰%李高峰%李光淳%宋成%杨小玉
閆鵬%鄭偉東%張季凱%譚雲冰%李高峰%李光淳%宋成%楊小玉
염붕%정위동%장계개%담운빙%리고봉%리광순%송성%양소옥
组织构建%神经组织构建%骶神经根%刺激电极%骶神经%髓鞘%应用%细胞凋亡%省级基金
組織構建%神經組織構建%骶神經根%刺激電極%骶神經%髓鞘%應用%細胞凋亡%省級基金
조직구건%신경조직구건%저신경근%자격전겁%저신경%수초%응용%세포조망%성급기금
背景:有研究表明基于阳极阻滞技术的骶神经根刺激器能有效重建脊髓损伤兔的膀胱排尿功能,但符合此技术的刺激电极至今未见报道。<br> 目的:设计并研制既与兔骶神经根匹配又符合阳极阻滞技术的刺激电极,观察长期植入刺激电极的兔骶神经根超微结构及病理形态学变化,评估刺激电极安全性。<br> 方法:纳入新西兰兔30只,随机抽取10只兔切取双侧S 2及S 3神经前根,光镜下测量其直径后,制成与其直径相匹配的套筒型刺激电极。将剩余20只兔随机分为对照组及植入组,每组10只。植入组麻醉后将刺激电极植入S2及S3神经根前处,饲养半年后处死取材,观察植入处骶神经根超微结构变化。<br> 结果与结论:长期植入该刺激电极后,光学显微镜下见植入组植入处骶神经根神经细胞结构保存良好,轴突无明显变性,无炎症细胞浸润及胶质瘢痕形成;透射电镜下观察,植入组髓鞘排列紧密,无脱髓鞘现象,神经元无核萎缩、核凹陷和异染色质增多等现象。免疫组织化学染色显示,与对照组相比,植入组植入处神经根中胶质纤维酸性蛋白、Bax,Bcl-2和Caspase-3蛋白表达差异无显著性意义。结果说明实验成功研制了兔骶神经根刺激电极,长期植入骶神经根未出现组织病理学改变及无细胞凋亡现象,安全性好。
揹景:有研究錶明基于暘極阻滯技術的骶神經根刺激器能有效重建脊髓損傷兔的膀胱排尿功能,但符閤此技術的刺激電極至今未見報道。<br> 目的:設計併研製既與兔骶神經根匹配又符閤暘極阻滯技術的刺激電極,觀察長期植入刺激電極的兔骶神經根超微結構及病理形態學變化,評估刺激電極安全性。<br> 方法:納入新西蘭兔30隻,隨機抽取10隻兔切取雙側S 2及S 3神經前根,光鏡下測量其直徑後,製成與其直徑相匹配的套筒型刺激電極。將剩餘20隻兔隨機分為對照組及植入組,每組10隻。植入組痳醉後將刺激電極植入S2及S3神經根前處,飼養半年後處死取材,觀察植入處骶神經根超微結構變化。<br> 結果與結論:長期植入該刺激電極後,光學顯微鏡下見植入組植入處骶神經根神經細胞結構保存良好,軸突無明顯變性,無炎癥細胞浸潤及膠質瘢痕形成;透射電鏡下觀察,植入組髓鞘排列緊密,無脫髓鞘現象,神經元無覈萎縮、覈凹陷和異染色質增多等現象。免疫組織化學染色顯示,與對照組相比,植入組植入處神經根中膠質纖維痠性蛋白、Bax,Bcl-2和Caspase-3蛋白錶達差異無顯著性意義。結果說明實驗成功研製瞭兔骶神經根刺激電極,長期植入骶神經根未齣現組織病理學改變及無細胞凋亡現象,安全性好。
배경:유연구표명기우양겁조체기술적저신경근자격기능유효중건척수손상토적방광배뇨공능,단부합차기술적자격전겁지금미견보도。<br> 목적:설계병연제기여토저신경근필배우부합양겁조체기술적자격전겁,관찰장기식입자격전겁적토저신경근초미결구급병리형태학변화,평고자격전겁안전성。<br> 방법:납입신서란토30지,수궤추취10지토절취쌍측S 2급S 3신경전근,광경하측량기직경후,제성여기직경상필배적투통형자격전겁。장잉여20지토수궤분위대조조급식입조,매조10지。식입조마취후장자격전겁식입S2급S3신경근전처,사양반년후처사취재,관찰식입처저신경근초미결구변화。<br> 결과여결론:장기식입해자격전겁후,광학현미경하견식입조식입처저신경근신경세포결구보존량호,축돌무명현변성,무염증세포침윤급효질반흔형성;투사전경하관찰,식입조수초배렬긴밀,무탈수초현상,신경원무핵위축、핵요함화이염색질증다등현상。면역조직화학염색현시,여대조조상비,식입조식입처신경근중효질섬유산성단백、Bax,Bcl-2화Caspase-3단백표체차이무현저성의의。결과설명실험성공연제료토저신경근자격전겁,장기식입저신경근미출현조직병이학개변급무세포조망현상,안전성호。
BACKGROUND:Studies have shown that sacral nerve-root stimulation based on anodes block technique can effectively reconstruct the bladder voiding function of the rabbits with spinal cord injury. But the corresponding technology of stimulating electrode has not been reported so far. <br> OBJECTIVE:To design and develop the stimulating electrodes matching with both rabbit sacral nerve roots and anodal blocking technique, to observe the ultrastructure and morphological change of rabbit sacral nerve roots which implanted in electrode stimulation for a long-term and to assess the safety of stimulating electrodes. <br> METHODS:Thirty New Zealand rabbits were included, 10 rabbits were randomly selected from them and sacrificed after anesthesia, and then cut the anterior roots of bilateral S 2 and S 3 immediately;after measuring the diameter under the light microscope, the sleeve type stimulation electrode matched with the diameter was made. The remaining 20 rabbits were randomly divided into control group and implantation group, with 10 rabbits in each group. In the implantation group, the stimulating electrodes were implanted into the forepart of S 2 and S 3 nerve roots after anesthesia, and then sacrificed after fed for half a year for col ecting the samples. Then ultrastructure change of sacral nerve roots with the implantation was observed. <br> RESULTS AND CONCLUSION:Structure of nerve cel s of sacral nerve roots remained in good condition under a light microscope after long-term implantation of the stimulating electrodes. No obvious degeneration of axons, no inflammatory infiltration and glial scar formation were observed. In the implantation group, myelins arranged closely without demyelination phenomenon, and there was no atrophy of neuronal nuclear, no nuclear sag, no increased nuclear decompression and heterochromatin in neurons under the light microscope. Immunohistochemical analysis showed, compared with the control group, there were no significant differences in the expressions of glial fibril ary acidic protein, Bax, Bcl-2 and Caspase-3 proteins of nerve roots in the implantation group. The stimulation electrode of rabbit sacral nerve root is developed successful y, that is, the implantation is simple and safe as it can be used for long-term implantation without histopathological changes and apoptosis.