中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
37期
6580-6586
,共7页
郭华%夏璐%周君%陈世菊%何成奇
郭華%夏璐%週君%陳世菊%何成奇
곽화%하로%주군%진세국%하성기
组织构建%软骨组织构建%骨关节炎,膝关节%软骨细胞%超声%细胞凋亡%caspase-3%caspase-8%基因
組織構建%軟骨組織構建%骨關節炎,膝關節%軟骨細胞%超聲%細胞凋亡%caspase-3%caspase-8%基因
조직구건%연골조직구건%골관절염,슬관절%연골세포%초성%세포조망%caspase-3%caspase-8%기인
背景:超声治疗能够缓解疼痛,改善膝骨关节炎患者的运动功能,但目前关于超声治疗的文献缺乏一致性。<br> 目的:进一步验证超声治疗的膝骨关节炎有效性。<br> 方法:24只兔子随机分为3组,正常组不干预;模型组兔行前交叉韧带离断诱导膝骨关节炎,不接受任何治疗;超声组兔建模后接受超声波治疗10 min/次,1次/d,0.3 W/cm2伊红染色进行兔关节软骨组织学观察,运用免疫印迹和 RT-PCR技术检测兔软骨中的caspases3和caspases8的表达,TUNEL技术检测兔膝骨关节软骨细胞凋亡率。,1 MHz,共治疗10次。采用苏木精-<br> 结果与结论:正常兔软骨组织软骨细胞呈柱状整齐的排列,模型中软骨层变薄,软骨细胞排列无序而少。超声治疗后软骨细胞重新排列,趋于整齐,细胞数增加。模型组为膝骨关节炎模型;与正常组比较,模型组和超声组改良Mankin 评分较高,模型组和超声组软骨细胞凋亡指数更高,模型组高于超声组。与正常组比较,模型组和超声组caspase-3和caspase-8表达较高,超声治疗后两项指标表达下降。结果表明,超声可以改善软骨组织结构、降低caspase-3、caspase -8的表达,降低软骨细胞凋亡。提示超声治疗膝骨关节炎是有效的。
揹景:超聲治療能夠緩解疼痛,改善膝骨關節炎患者的運動功能,但目前關于超聲治療的文獻缺乏一緻性。<br> 目的:進一步驗證超聲治療的膝骨關節炎有效性。<br> 方法:24隻兔子隨機分為3組,正常組不榦預;模型組兔行前交扠韌帶離斷誘導膝骨關節炎,不接受任何治療;超聲組兔建模後接受超聲波治療10 min/次,1次/d,0.3 W/cm2伊紅染色進行兔關節軟骨組織學觀察,運用免疫印跡和 RT-PCR技術檢測兔軟骨中的caspases3和caspases8的錶達,TUNEL技術檢測兔膝骨關節軟骨細胞凋亡率。,1 MHz,共治療10次。採用囌木精-<br> 結果與結論:正常兔軟骨組織軟骨細胞呈柱狀整齊的排列,模型中軟骨層變薄,軟骨細胞排列無序而少。超聲治療後軟骨細胞重新排列,趨于整齊,細胞數增加。模型組為膝骨關節炎模型;與正常組比較,模型組和超聲組改良Mankin 評分較高,模型組和超聲組軟骨細胞凋亡指數更高,模型組高于超聲組。與正常組比較,模型組和超聲組caspase-3和caspase-8錶達較高,超聲治療後兩項指標錶達下降。結果錶明,超聲可以改善軟骨組織結構、降低caspase-3、caspase -8的錶達,降低軟骨細胞凋亡。提示超聲治療膝骨關節炎是有效的。
배경:초성치료능구완해동통,개선슬골관절염환자적운동공능,단목전관우초성치료적문헌결핍일치성。<br> 목적:진일보험증초성치료적슬골관절염유효성。<br> 방법:24지토자수궤분위3조,정상조불간예;모형조토행전교차인대리단유도슬골관절염,불접수임하치료;초성조토건모후접수초성파치료10 min/차,1차/d,0.3 W/cm2이홍염색진행토관절연골조직학관찰,운용면역인적화 RT-PCR기술검측토연골중적caspases3화caspases8적표체,TUNEL기술검측토슬골관절연골세포조망솔。,1 MHz,공치료10차。채용소목정-<br> 결과여결론:정상토연골조직연골세포정주상정제적배렬,모형중연골층변박,연골세포배렬무서이소。초성치료후연골세포중신배렬,추우정제,세포수증가。모형조위슬골관절염모형;여정상조비교,모형조화초성조개량Mankin 평분교고,모형조화초성조연골세포조망지수경고,모형조고우초성조。여정상조비교,모형조화초성조caspase-3화caspase-8표체교고,초성치료후량항지표표체하강。결과표명,초성가이개선연골조직결구、강저caspase-3、caspase -8적표체,강저연골세포조망。제시초성치료슬골관절염시유효적。
BACKGROUND:Ultrasound therapy can relieve pain and improve the movement function in patients with knee osteoarthritis, but there lacks of consistency in the literatures of ultrasound therapy. <br> OBJECTIVE:To further identify the effectiveness of ultrasound therapy in the treatment of knee osteoarthritis. <br> METHODS:Twenty-four rabbits were randomly divided into three groups:normal group, model group and ultrasound group. The rabbit in the normal group received no intervention;rabbits in the model group received anterior cruciate ligament transaction to establish the knee osteoarthritis model without any treatment;the rabbit in the ultrasound group received ultrasound therapy after modeling for 10 minutes once time, once per day, 0.3 W/cm2 , 1 MHz and treated for 10 times. Hematoxylin-eosin staining was conducted for histological observation of rabbit articular cartilage;western blot and reverse transcription PCR assessment were used to assess the expressions of caspases-3 and caspases-8 in rabbit articular cartilage, while Terminal deoxynucleotidyl transferase dUTP nick end labeling was used to assess the ratio of chondrocytes apoptosis of rabbit knee articular cartilage. <br> RESULTS AND CONCLUSION:The normal rabbit cartilage tissues and chondrocytes were neatly arranged in column;the middle cartilage layer of the model was thin;the chondrocytes were arranged disorderly and became less. After ultrasound therapy, the chondrocytes were rearranged neatly, and the number was increased. Compared with the normal group, the Mankin scores in the model group and ultrasound group were higher;the apoptotic rate of chondrocytes was higher in the model group and ultrasound group than in the normal group, and was also higher in the model group than in the ultrasound group. Compared with the normal group, the expressions of caspases-3 and caspases-8 were higher in the model group and the ultrasound group, while decreased after ultrasound therapy. The results indicate that ultrasound can improve the structure of cartilage tissues, decrease the expressions of caspases-3 and caspases-8 and reduce the apoptosis rate of chondrocytes. It is effective for the treatment of knee osteoarthritis with ultrasound therapy.