CAJ | 학술논문

背景：高脂血症可导致大鼠骨质疏松，适量运动可增加骨密度，但短期运动对高脂血症导致骨质疏松的雄性动物骨密度的影响尚不清楚。 　　目的：探讨短期中等强度跑台运动对雄性高脂血症大鼠骨密度的影响。 　　方法：将26只SD雄性大鼠随机分为正常对照组(n=8)、高脂血症组(n=9)、运动干预组(n=9)。正常对照组喂饲普通饲料；其他两组喂饲高脂饲料建立大鼠高脂血症模型，持续4周。运动干预组大鼠进行跑台训练，每周训练5 d，共4周，每天强度为：第1周15 min，速度15 m/min；第2周20 min，速度15 m/min；后2周20 min，速度20 m/min，跑台坡度均为0°。实验结束后处死大鼠，取其右侧股骨检测骨密度、胫骨观察骨形态变化，测定血浆碱性磷酸酶活性和钙磷含量。 　　结果与结论：与正常对照组比较，高脂血症组股骨远端骨密度显著降低(P<0.05)，胫骨近端骨小梁排列稀疏变薄，间隙增宽，髓腔内有大量脂肪细胞浸润或融合成空泡，血浆碱性磷酸酶明显增加(P<0.01)。与高脂血症组比较，运动干预组股骨远端骨密度显著增加(P <0.05)，经体质量校正后股骨全骨密度增加(P <0.05)，胫骨近端骨小梁排列致密整齐，小梁间距小，血浆碱性磷酸酶增加(P<0.01)。血浆钙磷含量各组间差异无显著性意义。实验结果说明短期中等强度跑台运动增加高脂血症雄性大鼠骨密度。
배경：고지혈증가도치대서골질소송，괄량운동가증가골밀도，단단기운동대고지혈증도치골질소송적웅성동물골밀도적영향상불청초。 　　목적：탐토단기중등강도포태운동대웅성고지혈증대서골밀도적영향。 　　방법：장26지SD웅성대서수궤분위정상대조조(n=8)、고지혈증조(n=9)、운동간예조(n=9)。정상대조조위사보통사료；기타량조위사고지사료건립대서고지혈증모형，지속4주。운동간예조대서진행포태훈련，매주훈련5 d，공4주，매천강도위：제1주15 min，속도15 m/min；제2주20 min，속도15 m/min；후2주20 min，속도20 m/min，포태파도균위0°。실험결속후처사대서，취기우측고골검측골밀도、경골관찰골형태변화，측정혈장감성린산매활성화개린함량。 　　결과여결론：여정상대조조비교，고지혈증조고골원단골밀도현저강저(P<0.05)，경골근단골소량배렬희소변박，간극증관，수강내유대량지방세포침윤혹융합성공포，혈장감성린산매명현증가(P<0.01)。여고지혈증조비교，운동간예조고골원단골밀도현저증가(P <0.05)，경체질량교정후고골전골밀도증가(P <0.05)，경골근단골소량배렬치밀정제，소량간거소，혈장감성린산매증가(P<0.01)。혈장개린함량각조간차이무현저성의의。실험결과설명단기중등강도포태운동증가고지혈증웅성대서골밀도。
BACKGROUND:Some studies have shown that hyperlipidemia can lead to osteoporosis in rats, and exercise can increase the bone mineral density of rats. But the effect of short-time exercise on the bone mineral density of hyperlipdemia induced osteoporosis male rats is unclear yet. OBJECTIVE:To investigate the effect of short-time middle-load treadmil exercise on the bone mineral density of hyperlipidemia male rats. METHODS:Twenty-six male Sprague Dawley rats were randomly divided into three groups:control group (n=8), hyperlipidemia group (n=9) and exercise intervention group (n=9). The rats in the control group were fed with normal diet, and the rats in the other two groups were fed with high-fat diet and lasted for 4 weeks to establish the hyperlipidemia models. The rats in the exercise intervention group received treadmil exercise 5 days per week for 4 weeks according to the fol owing schedule:15 m/min for 15 minutes in the 1st week, 15 m/min for 20 minutes in the 2nd week, and then 20 m/min for 20 minutes in the last 2 weeks. Slope grade of the treadmil was adjusted at 0°. At the end of experiment, the rats were sacrificed, and the bone mineral density of the right femur, the morphological change of tibia, the level of plasma alkaline phosphates and calcium content were examined. RESULTS AND CONCLUSION:Compared with the control group, the distal femur bone mineral density in hyperlipidemia group was significantly decreased (P<0.05);histological analysis of the proximal tibia showed thinning and loss of bony trabeculae arrangement, the gap was widened, and a large amount of fat cel s infiltration or integration into vacuoles in the marrow was observed, the plasma alkaline phosphates was significantly increased (P<0.01). Compared with the hyperlipidemia group, distal femur bone mineral density in the exercise intervention group was increased (P<0.05). After adjust body weight, the whole femur bone mineral density was significantly greater in exercise intervention group compared to hyperlipidemia group (P<0.05). The histological analysis of the proximal tibia showed that the spaces of bone trabeculae decreased and the structure of bone trabeculae compacted, the alkaline phosphates activities were increased (P<0.01). There were no significant differences in serum calcium and phosphates levels between groups. The results show that short-time middle-load treadmil exercise can increase the bone mineral density of hyperlipidemia male rats.