CAJ | 학술논문

背景：前期研究表明,电穿孔介导的重组质粒pIRES-hBMP2-hVEGF165能促进牵引区早期新生血管的形成和新骨形成。 　　目的：观察不同时机转染基因对兔下颌骨牵张成骨过程中牵引区新骨生成的影响，探索基因导入的最佳转染时间，以获得更好的治疗效果。 　　方法：新西兰大白兔48只，全麻下行双侧下颌骨截骨及牵引器植入后，采用随机区组法分成4组，分别于造模后即刻、牵引开始时、牵引结束时在双侧牵引区注射2μg(0.1 g/L)重组质粒pIRES-hBMP2-hVEGF165，3组均予电穿孔刺激；单纯牵引组单纯牵引不行基因转染。各组于造模后3 d开始以0.8 mm/d、1次/d的速率进行牵引，连续牵引10 d；各组分别于固定期1，2，4，8周处死3只兔子，切取下颌牵引区新生组织行组织学检测和形态计量学分析。 　　结果与结论：组织学检查和形态计量学分析发现，牵引期转染组与即刻转染组、固定期转染组、单纯牵引组比较间隙内有更多的新生血管、成骨细胞和间充质细胞等成分，各时点新生骨量与新生骨小梁宽度明显高于后者(P <0.05)。表明在牵引开始时(牵引期)进行基因转染较其他时间转染促进新骨生成作用明显，能够获得最佳的促进新骨生成的效果，提示牵引期是下颌骨基因治疗的最佳时机。
배경：전기연구표명,전천공개도적중조질립pIRES-hBMP2-hVEGF165능촉진견인구조기신생혈관적형성화신골형성。 　　목적：관찰불동시궤전염기인대토하합골견장성골과정중견인구신골생성적영향，탐색기인도입적최가전염시간，이획득경호적치료효과。 　　방법：신서란대백토48지，전마하행쌍측하합골절골급견인기식입후，채용수궤구조법분성4조，분별우조모후즉각、견인개시시、견인결속시재쌍측견인구주사2μg(0.1 g/L)중조질립pIRES-hBMP2-hVEGF165，3조균여전천공자격；단순견인조단순견인불행기인전염。각조우조모후3 d개시이0.8 mm/d、1차/d적속솔진행견인，련속견인10 d；각조분별우고정기1，2，4，8주처사3지토자，절취하합견인구신생조직행조직학검측화형태계량학분석。 　　결과여결론：조직학검사화형태계량학분석발현，견인기전염조여즉각전염조、고정기전염조、단순견인조비교간극내유경다적신생혈관、성골세포화간충질세포등성분，각시점신생골량여신생골소량관도명현고우후자(P <0.05)。표명재견인개시시(견인기)진행기인전염교기타시간전염촉진신골생성작용명현，능구획득최가적촉진신골생성적효과，제시견인기시하합골기인치료적최가시궤。
BACKGROUND:The previous studies have shown that electroporation-mediated recombinant plasmid pIRES-hBMP2-hVEGF165 can promote the early angiogenesis and new bone formation in the traction zone. OBJECTIVE:To investigate the effect of gene transfection at different time on the new bone formation in the traction zone during mandibular distraction in rabbits, so as to explore the optimal time for gene therapy and obtain a better treatment effect. METHODS:Forty-eight New-Zealand rabbits were employed. After accomplished the bilateral mandibular osteotomy and distractor implantation under general anaesthesia, the rabbits were randomly divided into four groups. The bilateral traction zones were transfected with the 2μg (0.1g/L) recombinant plasmids pIRES-hBMP2-hVEGF165 at latency period, traction period and consolidation period, respectively, and the rabbits in these three groups received electroporation stimulation. The simple traction group only received traction without gene transfection. The rabbits in each group received traction at 3 days after modeling with the rate of 0.8 mm/d and once per day, and continuous traction for 10 days;three rabbits were sacrificed in each group at 1, 2, 4 and 8 week. The new tissues in the mandibular traction zone were cut for histological observation and histomorphometry analysis. RESULTS AND CONCLUSION:Histological examination and histomorphometry analysis showed that amounts of newly formed vessels, osteoblasts and mesenchymal cel s of distraction period transfection group were greater than those of the latency period transfection group, consolidation period transfection group and simple traction group, and the new bone volume and the width of bone trabecula in the distraction period transfection group were higher than those in the other three groups (P<0.05). It is better to transfect gene at the beginning of traction (distraction period) than at other stages of traction;in this way, we can obtain more remarkable effects on new bone formation. It suggests that the distraction stage is the optimal time for gene therapy.