中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
38期
6791-6796
,共6页
生物材料%纳米生物材料%纳米晶胶原基骨%骨髓单个核细胞%新骨形成%骨%颌骨缺损
生物材料%納米生物材料%納米晶膠原基骨%骨髓單箇覈細胞%新骨形成%骨%頜骨缺損
생물재료%납미생물재료%납미정효원기골%골수단개핵세포%신골형성%골%합골결손
背景：纳米晶胶原基骨复合骨髓单个核细胞可促进各种干细胞生长，诱导新骨形成和成血管化，促进最终成骨。<br> 目的：探讨骨髓单个核细胞复合纳米晶胶原基骨支架材料修复兔下颌骨缺损的可行性。<br> 方法：选择健康新西兰大白兔27只，制备新西兰大白兔双侧下颌骨人工制备骨缺损模型，分为3组，实验组骨缺损处植入自体骨髓单个核细胞复合纳米晶胶原基骨支架材料，对照组骨缺损处植入纳米晶胶原基骨支架材料，空白组骨缺损处不植入任何材料。术后4，8，12周制备组织标本，行大体观察、影像学分析、苏木精-伊红染色、扫描电镜检测。<br> 结果与结论：影像学检查及组织学染色显示，实验组骨缺损处愈合程度、成骨速度及质量明显优于其他组；扫描电镜显示实验组材料与骨接触紧密，组织相容性好，无炎症刺激反应；分析牙 CT 数据及新骨形成检测结果表明，实验组骨修复情况优于其他组(P<0.05)。表明骨髓单个核细胞复合纳米晶胶原基骨支架材料具有骨诱导和骨形成作用，可用于修复颌骨缺损。
揹景：納米晶膠原基骨複閤骨髓單箇覈細胞可促進各種榦細胞生長，誘導新骨形成和成血管化，促進最終成骨。<br> 目的：探討骨髓單箇覈細胞複閤納米晶膠原基骨支架材料脩複兔下頜骨缺損的可行性。<br> 方法：選擇健康新西蘭大白兔27隻，製備新西蘭大白兔雙側下頜骨人工製備骨缺損模型，分為3組，實驗組骨缺損處植入自體骨髓單箇覈細胞複閤納米晶膠原基骨支架材料，對照組骨缺損處植入納米晶膠原基骨支架材料，空白組骨缺損處不植入任何材料。術後4，8，12週製備組織標本，行大體觀察、影像學分析、囌木精-伊紅染色、掃描電鏡檢測。<br> 結果與結論：影像學檢查及組織學染色顯示，實驗組骨缺損處愈閤程度、成骨速度及質量明顯優于其他組；掃描電鏡顯示實驗組材料與骨接觸緊密，組織相容性好，無炎癥刺激反應；分析牙 CT 數據及新骨形成檢測結果錶明，實驗組骨脩複情況優于其他組(P<0.05)。錶明骨髓單箇覈細胞複閤納米晶膠原基骨支架材料具有骨誘導和骨形成作用，可用于脩複頜骨缺損。
배경：납미정효원기골복합골수단개핵세포가촉진각충간세포생장，유도신골형성화성혈관화，촉진최종성골。<br> 목적：탐토골수단개핵세포복합납미정효원기골지가재료수복토하합골결손적가행성。<br> 방법：선택건강신서란대백토27지，제비신서란대백토쌍측하합골인공제비골결손모형，분위3조，실험조골결손처식입자체골수단개핵세포복합납미정효원기골지가재료，대조조골결손처식입납미정효원기골지가재료，공백조골결손처불식입임하재료。술후4，8，12주제비조직표본，행대체관찰、영상학분석、소목정-이홍염색、소묘전경검측。<br> 결과여결론：영상학검사급조직학염색현시，실험조골결손처유합정도、성골속도급질량명현우우기타조；소묘전경현시실험조재료여골접촉긴밀，조직상용성호，무염증자격반응；분석아 CT 수거급신골형성검측결과표명，실험조골수복정황우우기타조(P<0.05)。표명골수단개핵세포복합납미정효원기골지가재료구유골유도화골형성작용，가용우수복합골결손。
BACKGROUND:Nano-hydroxyapatite/col agen combined with mononuclear cel s can promote the growth of a variety of stem cel s to induce the formation of new bone and vascularization, final y inducing osteogenesis. <br> OBJECTIVE:To investigate the feasibility of bone marrow mononuclear cel s combined with the nano-hydroxyapatite/col agen for repair of mandibular defects in a rabbit. <br> METHODS:Twenty-seven New Zealand white rabbits were selected to prepare bilateral mandibular bone defect models, and then divided into three groups. In experiment group, bone marrow mononuclear cel s combined with the nano-hydroxyapatite/col agen were implanted into mandibular defects;in control group, nano-hydroxyapatite/col agen scaffold was implanted;and in blank control group, nothing was implanted. Tissue specimens were prepared at weeks 4, 8, 12 for gross observation, imaging analysis, hematoxylin-eosin staining and scanning electron microscope. <br> RESULTS AND CONCLUSION:The imaging examination and histological staining showed that the bone quality and healing degree in the experimental group was better than those in the other groups. Scanning electron microscope showed that better histocompatibility and no inflammation reaction in the experimental group. Dental CT data showed that the experimental group had better repair effect than the other groups (P<0.05). These findings indicate that bone marrow mononuclear cel s combined with the nano-hydroxyapatite/col agen have capacity of bone induction and bone formation, which can be used to repair mandibular defects.
