CAJ | 학술논문

背景：目前胶原作为牙周组织工程支架材料仍具有机械强度差、降解速度快等缺点，将其与壳聚糖复合可改善上述问题。 　　目的：评估新型壳聚糖-胶原支架材料的体外生物相容性。 　　方法：通过 MTT 法评估100%，75%，50%，25%壳聚糖-胶原支架材料浸提液对人牙周膜细胞的毒性。选择第4-6代生长状态良好的人牙周膜细胞与壳聚糖-胶原支架共培养，观察细胞在支架上的生长情况，并检测与壳聚糖-胶原支架复合培养前后人牙周膜细胞碱性磷酸酶活性的变化。 　　结果与结论：新型壳聚糖-胶原支架具有双层结构，一侧表面致密，一侧表面疏松多孔。MTT 法检测不同浓度材料浸提液毒性评级为0或1级。扫描电子显微镜及组织学观察可见细胞在壳聚糖-胶原支架上增殖良好，且致密层可起屏障膜作用，阻挡细胞进入支架内部；复合培养24 h后，人牙周膜细胞的碱性磷酸酶活性与复合培养前无明显差异(P >0.05)，复合培养48，72 h后人牙周膜细胞的碱性磷酸酶活性高于复合培养前(P <0.05)。以上结果提示新型壳聚糖-胶原支架具有良好的生物相容性及屏障功能，可进一步应用于牙周组织工程的研究。
배경：목전효원작위아주조직공정지가재료잉구유궤계강도차、강해속도쾌등결점，장기여각취당복합가개선상술문제。 　　목적：평고신형각취당-효원지가재료적체외생물상용성。 　　방법：통과 MTT 법평고100%，75%，50%，25%각취당-효원지가재료침제액대인아주막세포적독성。선택제4-6대생장상태량호적인아주막세포여각취당-효원지가공배양，관찰세포재지가상적생장정황，병검측여각취당-효원지가복합배양전후인아주막세포감성린산매활성적변화。 　　결과여결론：신형각취당-효원지가구유쌍층결구，일측표면치밀，일측표면소송다공。MTT 법검측불동농도재료침제액독성평급위0혹1급。소묘전자현미경급조직학관찰가견세포재각취당-효원지가상증식량호，차치밀층가기병장막작용，조당세포진입지가내부；복합배양24 h후，인아주막세포적감성린산매활성여복합배양전무명현차이(P >0.05)，복합배양48，72 h후인아주막세포적감성린산매활성고우복합배양전(P <0.05)。이상결과제시신형각취당-효원지가구유량호적생물상용성급병장공능，가진일보응용우아주조직공정적연구。
BACKGROUND:At present, col agen as a material in periodontal tissue engineering has some disadvantages such as poor mechanical strength and rapid degradation speed. Col agen combined with chitosan can improve above-mentioned problems. OBJECTIVE:To evaluate the biocompatibility of a novel chitosan-col agen scaffold in vitro. METHODS:Cytotoxicity of the extract of chitosan-col agen scaffold in different concentrations (100%, 75%, 50%, 25%) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Human periodontal ligament cel s at 4-6 passages were cocultured with the chitosan-col agen scaffold. Cel growth on the scaffold was observed. Changes in alkaline phosphatase activity were detected in periodontal ligament cel s before and after coculture. RESULTS AND CONCLUSION:The novel chitosan-col agen scaffold was made up of double layers with one dense layer and another loose layer. The grade of the cytotoxicity of the scaffold was from 0 to 1. Scanning electron microscope and histological observation demonstrated that cel s grew wel on the chitosan-col agen scaffold;the dense layer could prevent cel s to migrate into the scaffold. There were no significant differences in alkaline phosphatase activity in human periodontal ligament cel s before and 24 hours after combined culture (P>0.05). Alkaline phosphatase activity in human periodontal ligament cel s was greatly higher at 48 and 72 hours after combined culture compared with that before culture (P<0.05). Above results indicated that the novel chitosan-col agen scaffold has a good biocompatibility and barrier function, and potential as a scaffold for periodontal tissue engineering.