CAJ | 학술논문

目的：探讨脂多糖(内毒素，LPS)联合人工喂养诱导新生大鼠坏死性小肠结肠炎(NEC)模型的方法并评价其效果。方法出生当日 SD 新生大鼠，按不同的造模方式随机分为9组（每组8只）：A 组大鼠人工喂养；B1、B2、B3、B4、B5、B6组为人工喂养+LPS 灌胃，LPS 的剂量分别为5、10、15、20、30、40 mg/kg，以筛选 LPS 诱导新生大鼠 NEC 模型的最佳剂量；C 组大鼠采取鼠乳喂养+LPS(30 mg/kg)灌胃；D 组大鼠为鼠乳喂养，作为正常对照组。每日定时称量体重。3天后禁食24小时取回肠末端组织 HE 染色，光镜下观察肠道组织病理损伤情况，并进行病理评分，评分≥2分确定为 NEC。按 B5组造模条件，重复两次，每次8只，分别为 B5a、B5b 组。结果 A、B4、B5、B6组新生大鼠均出现不同程度的活动减少，倦怠，进而可见腹胀及粪便颜色性状发生改变。实验结束 A、B 组体重不增或下降，C、D 组体重增加，A、B 组体重分别与 D 组比较，差异均有显著性(P ＜0.05)。各组病理评分分别为 A 组：1.78±0.52，B1组：1.76±0.32，B2组：1.83±0.1，B3组：1.87±0.33，B4组：2.16±0.11，B5组：3.34±0.37，B6组：3.78±0.51，C 组：0.52±0.42，D 组：0.3±0.48。B5a、B5b 组评分分别为3.3±0.17、3.35±0.44。B5、B6模型组分别与对照组比较，差异均有显著性(P<0.05)；模型组内 B5、B6组分别与 B1、B2、B3、B4组比较，差异均有显著性(P<0.05)；B5、B6组分别与 A 组比较，差异均有显著性(P<0.05)。B5a、B5b 分别与 B5组比较，差异均无显著性(P>0.05)。B4、B5、B6组的 NEC 发生率分别为25%（2/8）、75％(6/8)和100%（8/8）。A、B1、B2、B3、C、D组的 NEC 发生率均为0。结论在 LPS 剂量为30 mg/kg 时，联合人工喂养可诱导与人类新生儿 NEC临床特征、病理改变基本一致的严重肠道损伤，且其发生率高，重复性好，特异性高，可用于新生儿 NEC 的研究。目的：探討脂多糖(內毒素，LPS)聯閤人工餵養誘導新生大鼠壞死性小腸結腸炎(NEC)模型的方法併評價其效果。方法齣生噹日 SD 新生大鼠，按不同的造模方式隨機分為9組（每組8隻）：A 組大鼠人工餵養；B1、B2、B3、B4、B5、B6組為人工餵養+LPS 灌胃，LPS 的劑量分彆為5、10、15、20、30、40 mg/kg，以篩選 LPS 誘導新生大鼠 NEC 模型的最佳劑量；C 組大鼠採取鼠乳餵養+LPS(30 mg/kg)灌胃；D 組大鼠為鼠乳餵養，作為正常對照組。每日定時稱量體重。3天後禁食24小時取迴腸末耑組織 HE 染色，光鏡下觀察腸道組織病理損傷情況，併進行病理評分，評分≥2分確定為 NEC。按 B5組造模條件，重複兩次，每次8隻，分彆為 B5a、B5b 組。結果 A、B4、B5、B6組新生大鼠均齣現不同程度的活動減少，倦怠，進而可見腹脹及糞便顏色性狀髮生改變。實驗結束 A、B 組體重不增或下降，C、D 組體重增加，A、B 組體重分彆與 D 組比較，差異均有顯著性(P ＜0.05)。各組病理評分分彆為 A 組：1.78±0.52，B1組：1.76±0.32，B2組：1.83±0.1，B3組：1.87±0.33，B4組：2.16±0.11，B5組：3.34±0.37，B6組：3.78±0.51，C 組：0.52±0.42，D 組：0.3±0.48。B5a、B5b 組評分分彆為3.3±0.17、3.35±0.44。B5、B6模型組分彆與對照組比較，差異均有顯著性(P<0.05)；模型組內 B5、B6組分彆與 B1、B2、B3、B4組比較，差異均有顯著性(P<0.05)；B5、B6組分彆與 A 組比較，差異均有顯著性(P<0.05)。B5a、B5b 分彆與 B5組比較，差異均無顯著性(P>0.05)。B4、B5、B6組的 NEC 髮生率分彆為25%（2/8）、75％(6/8)和100%（8/8）。A、B1、B2、B3、C、D組的 NEC 髮生率均為0。結論在 LPS 劑量為30 mg/kg 時，聯閤人工餵養可誘導與人類新生兒 NEC臨床特徵、病理改變基本一緻的嚴重腸道損傷，且其髮生率高，重複性好，特異性高，可用于新生兒 NEC 的研究。
목적：탐토지다당(내독소，LPS)연합인공위양유도신생대서배사성소장결장염(NEC)모형적방법병평개기효과。방법출생당일 SD 신생대서，안불동적조모방식수궤분위9조（매조8지）：A 조대서인공위양；B1、B2、B3、B4、B5、B6조위인공위양+LPS 관위，LPS 적제량분별위5、10、15、20、30、40 mg/kg，이사선 LPS 유도신생대서 NEC 모형적최가제량；C 조대서채취서유위양+LPS(30 mg/kg)관위；D 조대서위서유위양，작위정상대조조。매일정시칭량체중。3천후금식24소시취회장말단조직 HE 염색，광경하관찰장도조직병리손상정황，병진행병리평분，평분≥2분학정위 NEC。안 B5조조모조건，중복량차，매차8지，분별위 B5a、B5b 조。결과 A、B4、B5、B6조신생대서균출현불동정도적활동감소，권태，진이가견복창급분편안색성상발생개변。실험결속 A、B 조체중불증혹하강，C、D 조체중증가，A、B 조체중분별여 D 조비교，차이균유현저성(P ＜0.05)。각조병리평분분별위 A 조：1.78±0.52，B1조：1.76±0.32，B2조：1.83±0.1，B3조：1.87±0.33，B4조：2.16±0.11，B5조：3.34±0.37，B6조：3.78±0.51，C 조：0.52±0.42，D 조：0.3±0.48。B5a、B5b 조평분분별위3.3±0.17、3.35±0.44。B5、B6모형조분별여대조조비교，차이균유현저성(P<0.05)；모형조내 B5、B6조분별여 B1、B2、B3、B4조비교，차이균유현저성(P<0.05)；B5、B6조분별여 A 조비교，차이균유현저성(P<0.05)。B5a、B5b 분별여 B5조비교，차이균무현저성(P>0.05)。B4、B5、B6조적 NEC 발생솔분별위25%（2/8）、75％(6/8)화100%（8/8）。A、B1、B2、B3、C、D조적 NEC 발생솔균위0。결론재 LPS 제량위30 mg/kg 시，연합인공위양가유도여인류신생인 NEC림상특정、병리개변기본일치적엄중장도손상，차기발생솔고，중복성호，특이성고，가용우신생인 NEC 적연구。
Objective To establish and evaluate the effect of the neonatal necrotizing enterocolitis (NEC) model induced by lipopolysaccharide (endotoxin, LPS) combined with formula feeding. Method According to different modeling methods, 0-day-postnatal SD rats were divided into 9 groups randomly, each group including 8 rats. Group A was given formula feeding alone; group B1, B2, B3, B4, B5 and B6 were given formula feeding and LPS intragastric administration, the dosages of LPS were respectively 5, 10, 15, 20, 30, 40 mg/kg in order to screen out the best one; group C was given breast feeding and LPS intragastric administration; group D was set up as control group and given breast feeding only. All rats were weighed every day at regular time. After 3 days feeding and fasting for 24 hours and then all the rats had been sacrificed. Terminal ileum were harvested and observed the intestinal histopathological damage by HE staining, and evaluated the ileal damage by pathological score. The rats with pathological score higher than two were defined as NEC. According to the modeling method of group B5, repeated twice, 8 rats each time, and divided into group B5a and B5b. Result Neonatal rats in group A, B4, B5 and B6 showed various degrees of physical inactivity, tiredness, and then abdominal distention and changes of stool’s color and character. On day 3, the weight of neonatal rats in group A and B had no increase or even loss, and group C and D had weight gain. The terminal weights and weight changes of group A and B were significantly different from that of Group C and D (P<0.05). The pathological score of every group were respectively: group A:1.78±0.52, group B1: 1.76±0.32, group B2: 1.83±0.1, group B3: 1.87±0.33, group B4: 2.16±0.11, group B5: 3.34±0.37, group B6: 3.78±0.51, group C: 0.52±0.42, group D: 0.3±0.48. There were significant differences between model groups B5, B6 and control group D (P<0.05). Among the model groups, the pathological scores of group B5, B6 were significantly higher than group B1, B2, B3 and B4 (P<0.05). There were significant differences between model groups B5, B6 and group A (P<0.05). There were no significant differences between model groups B5a, B5b and group B5. The incidences of NEC in group B4, B5 and B6 respectively were 25%(2/8), 75%(6/8) and100%(8/8). While the incidences of NEC in group A, B1, B2, B3, C and D were all zero.Conclusion When the dosage of LPS was 30mg/kg and combined with formula feeding could induced serious intestinal damage, which had almost the same clinical features and pathological changes as human neonatal NEC. This modeling method has high incidence, good repeatability and high specificity, which could be used in the research of neonatal NEC.