CAJ | 학술논문

目的：探讨抗核抗体（ANA）、抗双链DNA（dsDNA）抗体及抗可溶性抗原（ENA）抗体谱对系统性红斑狼疮（SLE）的临床诊断价值。方法将158例SLE患者作为SLE组，50例健康者作为对照组。分别采用间接免疫荧光法（IIF）及免疫印迹法（IBT ）对ANA、抗dsDNA抗体及抗ENA抗体谱进行检测。结果 ANA、抗dsDNA抗体及抗ENA抗体谱检测在SLE诊断中的阳性率分别为81．65％、68．35％及87．34％，均低于三者联合检测（93．04％），差异有统计学意义（ P＜0．05）。抗EN A抗体谱中，抗U1核糖核蛋白（U1-nRNP）抗体的阳性率（52．53％）最高。结论联合检测ANA、抗dsDNA抗体及抗ENA抗体谱对SLE的早期诊断具有一定的临床价值。
목적：탐토항핵항체（ANA）、항쌍련DNA（dsDNA）항체급항가용성항원（ENA）항체보대계통성홍반랑창（SLE）적림상진단개치。방법장158례SLE환자작위SLE조，50례건강자작위대조조。분별채용간접면역형광법（IIF）급면역인적법（IBT ）대ANA、항dsDNA항체급항ENA항체보진행검측。결과 ANA、항dsDNA항체급항ENA항체보검측재SLE진단중적양성솔분별위81．65％、68．35％급87．34％，균저우삼자연합검측（93．04％），차이유통계학의의（ P＜0．05）。항EN A항체보중，항U1핵당핵단백（U1-nRNP）항체적양성솔（52．53％）최고。결론연합검측ANA、항dsDNA항체급항ENA항체보대SLE적조기진단구유일정적림상개치。
Objective To investigate clinical value of the anti-nuclear antibody (ANA ) ,anti-double strand DNA (dsDNA ) anti-body and anti-extractable nuclear antigen(ENA) antibody repertoire in systemic lupus erythematosus (SLE) diagnosis .Methods 158 SLE patients were served as SLE group and another 50 healthy people as the control group .Indirect immunofluorescence(IIF) and immunoblotting test(IBT ) were employed to detect ANA ,anti-dsDNA antibody and anti-ENA antibody repertoire .Results Positive rates of ANA ,anti-dsDNA antibody and anti-ENA antibody repertoire in SLE diagnosis were 81 .65% ,68 .35% and 87 .34% ,respectively ,which were all lower than that of their combined detection (93 .04% ) ,with statistically significant difference (P<0 .05 ) .Among anti-ENA antibody repertoire ,the positive rate of anti-U1-nuclear ribonucleoprotein (U1-Nrnp ) antibody (52 .53% ) was the highest .Conclusion Combined detection of ANA ,anti-dsDNA antibody and anti-ENA antibodies repertoire has some clinical value of early diagnosis of SLE .