CAJ | 학술논문

目的：比较化学发光微粒子免疫分析法（CMIA）和酶联免疫吸附法（ELISA）用于乙型肝炎（乙肝）表面抗原检测的优势效果。方法选取2011年3月～2013年4月采用ELISA法与CMIA法对386例患者进行了乙肝表面抗原检测的对比研究。结果 CMIA法检测乙肝表面抗原的阳性率为51.3%，ELISA法阳性率为43.5%，CMIA法阳性率明显更高，与ELISA法比较差异有统计学意义（P<0.05）；CMIA法的灵敏度与特异性均明显高于ELISA法（P<0.05）。结论与ELISA法比较，CMIA法对乙肝表面抗原检测在阳性率、灵敏度以及特异性等方面均存在有比较明显的优势，故建议将CMIA法作为临床检测乙肝表面抗原的首选方法而推广应用。
목적：비교화학발광미입자면역분석법（CMIA）화매련면역흡부법（ELISA）용우을형간염（을간）표면항원검측적우세효과。방법선취2011년3월～2013년4월채용ELISA법여CMIA법대386례환자진행료을간표면항원검측적대비연구。결과 CMIA법검측을간표면항원적양성솔위51.3%，ELISA법양성솔위43.5%，CMIA법양성솔명현경고，여ELISA법비교차이유통계학의의（P<0.05）；CMIA법적령민도여특이성균명현고우ELISA법（P<0.05）。결론여ELISA법비교，CMIA법대을간표면항원검측재양성솔、령민도이급특이성등방면균존재유비교명현적우세，고건의장CMIA법작위림상검측을간표면항원적수선방법이추엄응용。
Objective To comparison of chemiluminescence immunoassay particles (Chemiluminescent Microparticle Immuno Assay,CMIA) and enzyme-linked immunosorbent (Enzyme-Linked ImmunoSorbent Assay,ELISA) for the advantage of hepatitis b (HBV) surface antigen detection results. Methods In the past two years by ELISA and CMIA method 386 cases patients were hepatitis B surface antigen were compared. Results CMIA assay for hepatitis B surface antigen positive rate 51.3%, ELISA method positive rate 43.5%, CMIA method was signiifcantly higher compared with the ELISA method was statistically signiifcant (P<0.05);CMIA The sensitivity and speciifcity were signiifcantly higher than that of the ELISA method (P<0.05). Conclusion Compared with ELISA method, CMIA method for detection of hepatitis b surface antigen in positive rate, sensitivity and speciifcity of existence has obvious advantages, therefore, proposed to CMIA method as the preferred method of clinical detection of hepatitis b surface antigen and popularization and application.