CAJ | 학술논문

目的：探讨胃癌细胞SGC7901中高表达的肿瘤易感基因101（TSG101）对肿瘤增殖的作用及机制。方法将不同TSG101表达水平的胃癌细胞亚系接种于裸鼠体内，观察移植瘤生长状况及相关因子的表达。结果 TSG101转染组裸鼠的移植瘤体积明显高于空质粒组与未转染组（P<0.05）。 TSG101转染组瘤组织切片中血管内皮细胞生长因子（VEGF）表达明显高于空质粒组和未转染组（P<0.05）。结论 SGC7901细胞中高表达的TSG101可能通过上调VEGF的表达增强胃癌细胞增殖。
목적：탐토위암세포SGC7901중고표체적종류역감기인101（TSG101）대종류증식적작용급궤제。방법장불동TSG101표체수평적위암세포아계접충우라서체내，관찰이식류생장상황급상관인자적표체。결과 TSG101전염조라서적이식류체적명현고우공질립조여미전염조（P<0.05）。 TSG101전염조류조직절편중혈관내피세포생장인자（VEGF）표체명현고우공질립조화미전염조（P<0.05）。결론 SGC7901세포중고표체적TSG101가능통과상조VEGF적표체증강위암세포증식。
Objective To explore the effect and mechanism of tumor susceptibility gene 101 (TSG101) of highly ex-pressed gastric cancer SGC7901 cell on tumor proliferation. Methods Cells were divided into non-transfection group, empty plasmid transfection group and TSG101 eukaryotic expression plasmid transfection group (the TSG101 transfection group). Then observed the growth conditions of transplantation tumor and the expression of correlation factor. Results The expression of VEGF of the TSG101 transfection group of tumor tissue in nude mice was significantly higher than that of the non-transfection group and the empty plasmid transfection group(P<0.05). The transplantation tumor volume of the TSG101 transfection group of tumor tissue in nude mice was significantly higher than that of the non-transfection group and the empty plasmid transfection group (P<0.05). Conclusion The high-expression of TSG101 in SGC7901 cells may enhance the proliferation of gastric cancer by up-regulate the expression of VEGF.