河北医药
河北醫藥
하북의약
HEBEI MEDICAL JOURNAL
2014年
11期
1623-1626
,共4页
口腔扁平苔藓%Bcl-2%Bax
口腔扁平苔蘚%Bcl-2%Bax
구강편평태선%Bcl-2%Bax
oral lichen planus%Bcl-2%Bax
目的：通过对口腔扁平苔藓病变发展过程中细胞凋亡状况和凋亡相关蛋白表达的研究，探讨扁平苔藓的发病机制。方法采用免疫组化SP法，分析正常口腔黏膜上皮25例，扁平苔藓组织25例中凋亡相关蛋白Bcl-2、Bax表达水平及分布差异。结果与正常黏膜比较，Bcl-2在口扁平苔藓固有层与上皮层均显过度表达（ P ＜0揪．05），随淋巴细胞聚集密度的增加，其在固有层表达强度也明显增强，并高于上皮层（ P ＜0．05）。正常黏膜Bax弱表达局限于角化层，口腔扁平苔藓中Bax表达较正常组明显增强，随糜烂程度的加重，Bax表达明显增强（ P ＜0．01），分布更广泛。结论 Bcl-2在扁平苔藓固有层中过度表达抑制了淋巴细胞的凋亡的，强化细胞免疫， Bax过度表达是对异常增殖的上皮细胞和免疫细胞的拮抗反应，试图通过诱导凋亡，恢复细胞数目的稳定和免疫的平衡，但是过度凋亡致糜烂萎缩皮损。
目的：通過對口腔扁平苔蘚病變髮展過程中細胞凋亡狀況和凋亡相關蛋白錶達的研究，探討扁平苔蘚的髮病機製。方法採用免疫組化SP法，分析正常口腔黏膜上皮25例，扁平苔蘚組織25例中凋亡相關蛋白Bcl-2、Bax錶達水平及分佈差異。結果與正常黏膜比較，Bcl-2在口扁平苔蘚固有層與上皮層均顯過度錶達（ P ＜0揪．05），隨淋巴細胞聚集密度的增加，其在固有層錶達彊度也明顯增彊，併高于上皮層（ P ＜0．05）。正常黏膜Bax弱錶達跼限于角化層，口腔扁平苔蘚中Bax錶達較正常組明顯增彊，隨糜爛程度的加重，Bax錶達明顯增彊（ P ＜0．01），分佈更廣汎。結論 Bcl-2在扁平苔蘚固有層中過度錶達抑製瞭淋巴細胞的凋亡的，彊化細胞免疫， Bax過度錶達是對異常增殖的上皮細胞和免疫細胞的拮抗反應，試圖通過誘導凋亡，恢複細胞數目的穩定和免疫的平衡，但是過度凋亡緻糜爛萎縮皮損。
목적：통과대구강편평태선병변발전과정중세포조망상황화조망상관단백표체적연구，탐토편평태선적발병궤제。방법채용면역조화SP법，분석정상구강점막상피25례，편평태선조직25례중조망상관단백Bcl-2、Bax표체수평급분포차이。결과여정상점막비교，Bcl-2재구편평태선고유층여상피층균현과도표체（ P ＜0추．05），수림파세포취집밀도적증가，기재고유층표체강도야명현증강，병고우상피층（ P ＜0．05）。정상점막Bax약표체국한우각화층，구강편평태선중Bax표체교정상조명현증강，수미란정도적가중，Bax표체명현증강（ P ＜0．01），분포경엄범。결론 Bcl-2재편평태선고유층중과도표체억제료림파세포적조망적，강화세포면역， Bax과도표체시대이상증식적상피세포화면역세포적길항반응，시도통과유도조망，회복세포수목적은정화면역적평형，단시과도조망치미란위축피손。
Objective To explore the pathogenesis of oral lichen planus ( OLP) through examining the expression of apoptosis-associated protein Bcl-2 and Bax in OLP .Methods The expression levels and distribution of Bcl-2 and Bax were detected by immunohistochemistry(SP) in 25 cases of normal mucosa and 25 cases OLP tissues.Results As compared with that in normal mucosa,overexpression of Bcl-2 was found in lamina propria and epithelial lining of OLP ( P <0.05),the expression levels of Bcl-2 in lamina propria of OLP were increased with the increase of lymphocyte aggregation dencity ,which were significantly higher than those in epithelial lining of OLP ( P <0.05).The weak expression of Bax in normal mucosa was limited in cuticular layer , however , the expression levels of Bax were increased , as compared with those in normal mucosa , moreover,which were further increased with the aggravation of erosion degree ( P <0.01),and its distribution was more wide. Conclusion The overexpression of Bcl-2 in lamina propria of OLP inhibits apoptosis of lymphocytes and strengthens cellular immunity .The overexpression of Bax is antagonism reaction to epithelial cells and immunocyte of abnormity proliferation ,which try to recover stabilization of cell number and immune balance by inducing cell apoptosis ,however,excess apoptosis can result in erosion,atrophy,skin injury in OLP.
