CAJ | 학술논문

目的：用国产试剂取代原装试剂并统计得出L-8900氨基酸分析仪血浆氨基酸生物参考区间，为临床诊断提供服务。方法用国产试剂与原装试剂检测标准品和同一批血浆（50例），并进行比较，观察其性能指标（包括分离度和重复性）及准确度；使用国产试剂检测400例健康人的血浆游离氨基酸水平以建立血浆氨基酸生物参考区；同时与肝功能指标进行相关性分析。结果（1）连续5次检测氨基酸标准品得出其峰分离度高，峰保留时间和峰面积重复性高，国产试剂准确度好。（2）统计得出19种氨基酸的生物参考区间，其中10种氨基酸存在性别差异。（3）丙氨酸氨基转移酶（ALT）水平与苏氨酸水平呈负相关（P＜0．05），血糖（GLU）水平与缬氨酸、异亮氨酸、亮氨酸水平呈正相关（P＜0．05），总胆固醇（CHO）水平与异亮氨酸水平呈负相关（P＜0．05）。结论国产试剂可以替代原装试剂，在此基础上得出血浆氨基酸生物参考区间对临床诊断及预后具有重要意义。
목적：용국산시제취대원장시제병통계득출L-8900안기산분석의혈장안기산생물삼고구간，위림상진단제공복무。방법용국산시제여원장시제검측표준품화동일비혈장（50례），병진행비교，관찰기성능지표（포괄분리도화중복성）급준학도；사용국산시제검측400례건강인적혈장유리안기산수평이건립혈장안기산생물삼고구；동시여간공능지표진행상관성분석。결과（1）련속5차검측안기산표준품득출기봉분리도고，봉보류시간화봉면적중복성고，국산시제준학도호。（2）통계득출19충안기산적생물삼고구간，기중10충안기산존재성별차이。（3）병안산안기전이매（ALT）수평여소안산수평정부상관（P＜0．05），혈당（GLU）수평여힐안산、이량안산、량안산수평정정상관（P＜0．05），총담고순（CHO）수평여이량안산수평정부상관（P＜0．05）。결론국산시제가이체대원장시제，재차기출상득출혈장안기산생물삼고구간대림상진단급예후구유중요의의。
Objective To calculate the biological reference of plasma amino acid on L-8900 amino acid analyzer and to provide reference for clinical diagnosis with domestic reagents replacing original reagent .Methods By testing the original standards and the same batch of plasma (50 cases) ,we compared original reagents with domestic reagents for their performance (including resolution , repeatability ,accuracy ) .We tested the plasma free amino acids of 400 cases of healthy people using the domestic reagents to estab-lish biological reference interval of plasma amino acid ,and do correlation analysis between the amino acids level and liver function . Results (1)Domestic reagents showed high accuracy in the results of 5 consecutive detection of amino acids were high peak separa-tion and high peak retention time and high peak area .(2)Statistics derived biological reference interval of 19 amino acids and 10 kinds of amino acids had significant differences .(3)Correlation analysis showed that ALT and liver function were negatively corre-lated with threonine(P<0 .05) .GLU and valine ,isoleucine ,leucine were positively correlated(P<0 .05) .CHO and negatively cor-related with isoleucine(P<0 .05) .Conclusion Domestic agents can replace the original reagents ,on the basis ,the biological refer-ence intervals of plasma amino acids have great importance to clinical diagnosis and prognosis .