CAJ | 학술논문

目的建立一种快速、灵敏和可靠的气相色谱法以评价氟康唑原位胶化眼部给药系统兔眼应用后氟康唑的生物利用度.方法应用大口径毛细管柱.检测为氮磷检测器.将泪液处理后和房水用甲醇溶解注入色谱仪中.采用外标法定量.结果每个样品的分析时间小于10min,最小可检测浓度为0.01μg/ml,在0.1至20.0μg/ml范围内线性关系良好.药物从泪液和房水中的回收率分别为99.0%和94.8%.结论此方法可用于测定兔眼泪液和房水中的氟康唑浓度,对于评价氟康唑局部应用后眼部生物利用度是一个可行的、快速的、有效可靠的方法.
목적건립일충쾌속、령민화가고적기상색보법이평개불강서원위효화안부급약계통토안응용후불강서적생물이용도.방법응용대구경모세관주.검측위담린검측기.장루액처리후화방수용갑순용해주입색보의중.채용외표법정량.결과매개양품적분석시간소우10min,최소가검측농도위0.01μg/ml,재0.1지20.0μg/ml범위내선성관계량호.약물종루액화방수중적회수솔분별위99.0%화94.8%.결론차방법가용우측정토안루액화방수중적불강서농도,대우평개불강서국부응용후안부생물이용도시일개가행적、쾌속적、유효가고적방법.
Objective　To develop a rapid，sensitive and reliable high-performance gas chromatography to evaluate the fluconazole (FCZ) bioavailability after topically applied FCZ in-situ gelling ophthalmic delivery system to the rabbit eyes.Methods　A megabore capillary column（30m long and inner diameter of 0.530 mm） with a 0.88-μm-thick bonded liquid phase was used.Detector was nitrogen-selective type.Tears adsorbed to the filter paper was directly by methanol with no evaporation stage prior to analysis.Aqueous humors was extracted by ethyl acetate.Results　The duration of each analysis was less 10 min and the minimum detectable concentration was 0.01μg/ml.The assay was linear from 0.1 to 20 μg/ml.The average recoveries from tears and aqueous humors were 99.0% and 94.8%，respectively.Conclusion　This method can be used to determine rabbit tears and aqueous humors levels of fluconazole and was practicable approach for evaluating intraocular pharmacokinetics after topically applied to rabbit eyes.