CAJ | 학술논문

目的:观察microRNA-21(miR-21)在缺血再灌注损伤(I/R)早期大鼠心肌的表达及对细胞凋亡的影响.方法:SD大鼠随机分为对照组(冠状动脉注射转染空白对照病毒rAAV9-ZsGreen),miR-21组(冠状动脉注射转染rAAV9-ZsGreen-pre-miR-21),Sham组(仅开胸挂线),I/R组(进行I/R处理),I/R+miR-21(转染rAAV9-ZsGreen-pre-miR-21后再进行I/R处理).用Realtime-PCR检测大鼠miR-21水平,免疫组织化学及Western印迹方法检测Bd-2,Bax,caspase-3水平并计算Bcl-2/Bax比值.结果:冠状动脉注射rAAV9-ZsGreen-pre-miR-21可使miR-21表达增加4.43倍(P＜0.001);与Sham组比较,I/R组大鼠缺血区miR-21水平下调(P＜0.05),而非缺血区miR-21升高(P＜0.05);与Sham组比较,I/R组再灌注1,2,6hmiR-21水平进行性下降(P＜0.05),在同一时间点I/R+miR-21组较I/R组miR-21表达升高(P＜0.05);与Sham组比较,再灌注6h时I/R及I/R+miR-21组Bcl-2增高、caspase-3增高、Bcl-2/Bax降低(P＜0.05),与I/R组比较,I/R+miR-21组Bcl-2下调、caspase-3下调、Bcl-2/Bax增高(P＜0.05).结论:I/R早期大鼠心肌缺血区miR-21表达下降,细胞凋亡增加,过表达miR-21可改善细胞凋亡.
목적:관찰microRNA-21(miR-21)재결혈재관주손상(I/R)조기대서심기적표체급대세포조망적영향.방법:SD대서수궤분위대조조(관상동맥주사전염공백대조병독rAAV9-ZsGreen),miR-21조(관상동맥주사전염rAAV9-ZsGreen-pre-miR-21),Sham조(부개흉괘선),I/R조(진행I/R처리),I/R+miR-21(전염rAAV9-ZsGreen-pre-miR-21후재진행I/R처리).용Realtime-PCR검측대서miR-21수평,면역조직화학급Western인적방법검측Bd-2,Bax,caspase-3수평병계산Bcl-2/Bax비치.결과:관상동맥주사rAAV9-ZsGreen-pre-miR-21가사miR-21표체증가4.43배(P＜0.001);여Sham조비교,I/R조대서결혈구miR-21수평하조(P＜0.05),이비결혈구miR-21승고(P＜0.05);여Sham조비교,I/R조재관주1,2,6hmiR-21수평진행성하강(P＜0.05),재동일시간점I/R+miR-21조교I/R조miR-21표체승고(P＜0.05);여Sham조비교,재관주6h시I/R급I/R+miR-21조Bcl-2증고、caspase-3증고、Bcl-2/Bax강저(P＜0.05),여I/R조비교,I/R+miR-21조Bcl-2하조、caspase-3하조、Bcl-2/Bax증고(P＜0.05).결론:I/R조기대서심기결혈구miR-21표체하강,세포조망증가,과표체miR-21가개선세포조망.