食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2013年
5期
1445-1451
,共7页
李红权%孙良娟%吴晓薇%欧安%刘中勇
李紅權%孫良娟%吳曉薇%歐安%劉中勇
리홍권%손량연%오효미%구안%류중용
环介导等温扩增技术%食源性致病菌%检测应用
環介導等溫擴增技術%食源性緻病菌%檢測應用
배개도등온확증기술%식원성치병균%검측응용
loop-mediated isothermal amplification%foodborne pathogens%application in detection
环介导等温扩增(LAMP)技术是一种新型核酸扩增技术，该技术在60～65℃恒温条件1 h内，把特异性靶序列扩增到109水平，具有高特异性、高敏感性、简单、便捷及成本低的特点，已广泛应用于流行性细菌、病毒的定性定量检测等领域。本研究对 LAMP 的原理、特性及其应用做一综述，从而为其在食源性致病菌检测应用中提供理论依据。
環介導等溫擴增(LAMP)技術是一種新型覈痠擴增技術，該技術在60～65℃恆溫條件1 h內，把特異性靶序列擴增到109水平，具有高特異性、高敏感性、簡單、便捷及成本低的特點，已廣汎應用于流行性細菌、病毒的定性定量檢測等領域。本研究對 LAMP 的原理、特性及其應用做一綜述，從而為其在食源性緻病菌檢測應用中提供理論依據。
배개도등온확증(LAMP)기술시일충신형핵산확증기술，해기술재60～65℃항온조건1 h내，파특이성파서렬확증도109수평，구유고특이성、고민감성、간단、편첩급성본저적특점，이엄범응용우류행성세균、병독적정성정량검측등영역。본연구대 LAMP 적원리、특성급기응용주일종술，종이위기재식원성치병균검측응용중제공이론의거。
Loop-mediated isothermal amplification (LAMP) technology is a new technology for nucleic acid amplification, which can amplify the specific target sequences to 109 level under the conditions of constant temperature at 60~65℃within 1h. It has the characteristics of high specificity, sensitivity, simplicity, conven-ience and low cost. LAMP has been widely used in qualitative and quantitative detection of bacterial and viral epidemic.This article reviewed the principle, characteristics and application of LAMP technology, in order to provide theoretical basis for its application in the detection of foodborne pathogens.
