CAJ | 학술논문

目的：建立高压液相液相色谱法(HPLC)测定柑橘类水果中柑橘红2号染料的方法。方法样品用乙腈提取, NH2固相萃取小柱净化后,用Waters Sunfire C18(250 mm×4.6 mm,5.0μm)色谱柱进行分离,水-乙腈作为流动相,进行梯度洗脱,流速为1.0 mL/min.结果方法的线性范围为：0.5~5.0μg/mL (r=0.99973),检出限为0.003 mg/kg,定量限为0.01 mg/kg。在6个不同种类的柑橘中,添加柑橘红2号染料浓度为0.1、0.5、2.0 mg/kg 3个水平,回收率在79.48%~93.20%之间,相对标准偏差为6.5%~14.5%。利用该方法对市场上随机采集的36份柑橘样品进行了检测,其中,3份样品有检出。结论该方法灵敏度高,准确度好,适用于对柑橘类样品中柑橘红2号染料的检测。
목적：건립고압액상액상색보법(HPLC)측정감귤류수과중감귤홍2호염료적방법。방법양품용을정제취, NH2고상췌취소주정화후,용Waters Sunfire C18(250 mm×4.6 mm,5.0μm)색보주진행분리,수-을정작위류동상,진행제도세탈,류속위1.0 mL/min.결과방법적선성범위위：0.5~5.0μg/mL (r=0.99973),검출한위0.003 mg/kg,정량한위0.01 mg/kg。재6개불동충류적감귤중,첨가감귤홍2호염료농도위0.1、0.5、2.0 mg/kg 3개수평,회수솔재79.48%~93.20%지간,상대표준편차위6.5%~14.5%。이용해방법대시장상수궤채집적36빈감귤양품진행료검측,기중,3빈양품유검출。결론해방법령민도고,준학도호,괄용우대감귤류양품중감귤홍2호염료적검측。
Objective To establish a rapid method for the determination of citrus red 2 (CR2) in citrus by high pressure liquid chromatography (HPLC). Methods The samples were extracted with acetonitrile, cleaned-up by NH2 solid phase extraction cartridge. Then, the samples were separated on a Waters Sunfire C18 (250 mm×4.6 mm, 5.0 μm) in gradient elution with the mobile phases of water and acetonitrile. The separated compounds were detected with UV/VIS detector. The linearity of the method (r=0.99973) was good over the concentration range from 0.5 to 5.0 μg/mL for CR2. The limit of detection (LOD) was 0.003 mg/kg, and the limit of quantification (LOQ) was 0.01 mg/kg. Results The average recoveries of CR2 at 3 levels of 0.1, 0.5 and 2.0 mg/kg were between 79.48%and 93.20%with the relative standard deviations (RSDs) between 6.5%and 14.5%. A total of 36 citrus samples randomly collected from different markets were detected by the pro-posed method. The results showed that 3 samples contained citrus red 2. Conclusion The sensitivity, accuracy, and precision of this method could meet the requirements of the CR2 analysis.