CAJ | 학술논문

目的：评价荧光定量PCR技术在结核病诊断中的应用价值。方法：采用荧光定量PCR（FQ-PCR）、涂片法和培养法同时检测580例结核患者、疑似结核患者的痰、胸腹水、脑脊液标本，对检测结果进行比较。结果：荧光定量PCR检测阳性率要高于涂片法和培养法的阳性率，统计分析有显著性（P＜0.01）。结论：应用荧光定量PCR方法检测结核杆菌，具有特异、灵敏、简便、快速的特点，可作为结核病诊断的常规方法。
목적：평개형광정량PCR기술재결핵병진단중적응용개치。방법：채용형광정량PCR（FQ-PCR）、도편법화배양법동시검측580례결핵환자、의사결핵환자적담、흉복수、뇌척액표본，대검측결과진행비교。결과：형광정량PCR검측양성솔요고우도편법화배양법적양성솔，통계분석유현저성（P＜0.01）。결론：응용형광정량PCR방법검측결핵간균，구유특이、령민、간편、쾌속적특점，가작위결핵병진단적상규방법。
Objective:To investigate clinical value of fluorescence quantitative PCR (FQ-PCR)on detection of mycobacterium tuberculosis complex(MTB).Methods:580 sputum, cerebrospinal fluid(SCF),hydrothorax and ascite samples from clinical MTB and suspected patients were detected by FQ-PCR,acid-fast staining and culture respectively.Sensitivity and specificity of FQ-PCR were assessed by comparing test results.Results:The Positive rate of FQ-PCR in testing samples was higher than that of acid -fast staining and culture,and there was a significant difference(P<0.01).Conclusion:FQ-PCR characterized by distinct specificity,high sensitivity, simplicity and quick-response can be used as a conventional method to diagnose of tuberculosis.