北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2013年
10期
7-8,9
,共3页
高效液相色谱法%锁阳%熊果酸
高效液相色譜法%鎖暘%熊果痠
고효액상색보법%쇄양%웅과산
HPLC%Cynomorium%Ursolic
以高效液相色谱法测定锁阳中熊果酸含量。采用WondaSil C18分析柱4.6nm×150nm，5μm，流动相：甲醇-0.5%磷酸水溶液-乙腈（80∶15∶5），流速：1ml/min；柱温：35℃；检测波长210nm，外标法定量。熊果酸的保留时间为15.239min。熊果酸进样量在0.841～8.096mg/mL范围内呈良好线性关系，其中熊果酸平均回收率为93.90%～98.78%，RSD为1.8%（n=6）。测得锁阳中熊果酸含量为0.466%～1.034%，此分析方法简单快捷，高效灵敏，重现性好。
以高效液相色譜法測定鎖暘中熊果痠含量。採用WondaSil C18分析柱4.6nm×150nm，5μm，流動相：甲醇-0.5%燐痠水溶液-乙腈（80∶15∶5），流速：1ml/min；柱溫：35℃；檢測波長210nm，外標法定量。熊果痠的保留時間為15.239min。熊果痠進樣量在0.841～8.096mg/mL範圍內呈良好線性關繫，其中熊果痠平均迴收率為93.90%～98.78%，RSD為1.8%（n=6）。測得鎖暘中熊果痠含量為0.466%～1.034%，此分析方法簡單快捷，高效靈敏，重現性好。
이고효액상색보법측정쇄양중웅과산함량。채용WondaSil C18분석주4.6nm×150nm，5μm，류동상：갑순-0.5%린산수용액-을정（80∶15∶5），류속：1ml/min；주온：35℃；검측파장210nm，외표법정량。웅과산적보류시간위15.239min。웅과산진양량재0.841～8.096mg/mL범위내정량호선성관계，기중웅과산평균회수솔위93.90%～98.78%，RSD위1.8%（n=6）。측득쇄양중웅과산함량위0.466%～1.034%，차분석방법간단쾌첩，고효령민，중현성호。
A determination method of ursolic acid in Cynomorium songaricum by reversed phase high performanceliquid chromatography was established. Using a WondaSail C18(4.6nm×150nm,5μm) column. The mobile phase consisted of methanol-0.5%phosphoric acid-acetonitrile(80∶15∶5),flow rate=1mL/min and temperature T=35℃.The detection wave length was at 210nm. With the range of 0.841~8.096μg for ursolic acid had good linearity. The average recovery was 96.54% , RSD=1.8%(n=6). The concentration of ursolic acid in Cynomorium songaricum at 0.466~1.034%, the method is higher accurate,simple and precise.
