北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2013年
10期
5-5,4
,共2页
高效液相色谱法%养胃颗粒%橙皮苷%含量
高效液相色譜法%養胃顆粒%橙皮苷%含量
고효액상색보법%양위과립%등피감%함량
HPLC%Yangwei Granules%Hesperidin%Assay%Determination
目的:建立高效液相色谱法测定养胃颗粒中橙皮苷含量。方法:采用Agela Venusil MP C18色谱柱(250mm×4.6mm,5μm),以甲醇-醋酸溶液(每1000mL水中含冰醋酸25mL)(35∶65)为流动相,流速为1.0mL/min,检测波长为284nm,柱温:30℃。结果:橙皮苷进样量在0.03508~0.5613μg(r=0.9999)范围内与峰面积积分值具有良好线性关系,平均加样回收率为98.87%,RSD为1.38%(n=6)。结论:该法简便、灵敏、准确可靠,可用于该药的含量测定。
目的:建立高效液相色譜法測定養胃顆粒中橙皮苷含量。方法:採用Agela Venusil MP C18色譜柱(250mm×4.6mm,5μm),以甲醇-醋痠溶液(每1000mL水中含冰醋痠25mL)(35∶65)為流動相,流速為1.0mL/min,檢測波長為284nm,柱溫:30℃。結果:橙皮苷進樣量在0.03508~0.5613μg(r=0.9999)範圍內與峰麵積積分值具有良好線性關繫,平均加樣迴收率為98.87%,RSD為1.38%(n=6)。結論:該法簡便、靈敏、準確可靠,可用于該藥的含量測定。
목적:건립고효액상색보법측정양위과립중등피감함량。방법:채용Agela Venusil MP C18색보주(250mm×4.6mm,5μm),이갑순-작산용액(매1000mL수중함빙작산25mL)(35∶65)위류동상,류속위1.0mL/min,검측파장위284nm,주온:30℃。결과:등피감진양량재0.03508~0.5613μg(r=0.9999)범위내여봉면적적분치구유량호선성관계,평균가양회수솔위98.87%,RSD위1.38%(n=6)。결론:해법간편、령민、준학가고,가용우해약적함량측정。
Objective:to establish a HPLC method for determination of the content of Hesperidin in Yangwei Granules. Methods:An Agela Venusil MP C18 column(250mm×4.6mm,5μm) was used with Methanol-Acetic Acid solution containing 25 mL of glacial acetic acid in 1000 mL of water (35∶65) as the mobile phase, the flow rate was 1.0mL/min, the detection wavelength was 284nm,and the column temperature was 30℃. Results:there was good linear relationship between the peak area and the concentrations in the range of 0.03508~0.5613μg (r=0.9999)for Hesperidin; the average spike recovery was 98.87% with RSD=1.38%(n=6). Conclusion:The method is simple, sensitive and accurate for the quality control of the product.