CAJ | 학술논문

目的检测牛黄清感胶囊对甲型A/Brisban/10/2008（H3N2）流感病毒抑制和预防的作用，为抗病毒药物的筛选、研发提供实验依据。方法在MDCK细胞培养内，采用病毒细胞形态变化CPE法、MTT染色法，检测不同浓度的牛黄清感胶囊对甲型A/Brisban/10/2008（H3N2）流感病毒的抑制和预防作用。实验分1次用药组和3次用药组，计算药物半数有效浓度IC50，最小有效浓度MIC及治疗指数TI。结果体外抑制甲型H3N2流感病毒实验中，“牛黄清感胶囊”单次用药组TI为27，三次用药组为TI为43。体外预防甲型H3N2流感病毒实验中，“牛黄清感胶囊”单次用药组TI为21.3，三次用药组为TI为27。结论体外抑制甲型A/Brisban/10/2008（H3N2）流感病毒的药效实验结果显示“牛黄清感胶囊”具有抑制和预防甲型流感病毒的作用。
목적검측우황청감효낭대갑형A/Brisban/10/2008（H3N2）류감병독억제화예방적작용，위항병독약물적사선、연발제공실험의거。방법재MDCK세포배양내，채용병독세포형태변화CPE법、MTT염색법，검측불동농도적우황청감효낭대갑형A/Brisban/10/2008（H3N2）류감병독적억제화예방작용。실험분1차용약조화3차용약조，계산약물반수유효농도IC50，최소유효농도MIC급치료지수TI。결과체외억제갑형H3N2류감병독실험중，“우황청감효낭”단차용약조TI위27，삼차용약조위TI위43。체외예방갑형H3N2류감병독실험중，“우황청감효낭”단차용약조TI위21.3，삼차용약조위TI위27。결론체외억제갑형A/Brisban/10/2008（H3N2）류감병독적약효실험결과현시“우황청감효낭”구유억제화예방갑형류감병독적작용。
Objective To detect the inhibition and prevention effect of Niuhuang Qinggan capsules for A/Brisban/10/2008 (H3N2) influenza virus A in order to provide experimental basis for the screening, research and development of antiviral drugs. Methods In the MDCK cells culture, the virus morphological change CPE method and MTT staining method were used to detect the inhibition and prevention effect of different concentrations of Niuhuang Qinggan capsules for A/Brisban/10/2008 (H3N2) influenza virus A. The experiment was consisted of the single-dose group and the three-dose. The drug medium effective concentration IC50, minimum effective concentration MIC and treatment index TI were calculated. Results In the experiment of vitro inhibitory effect of A H3N2 influenza, for the "Niuhuang Qinggan capsules", the T1 of single medicine group was 27. The T1 of the triple medicine group was 43. In the experiment of vitro preventive effect of A H3N2 influenza, for the "Niuhuang Qinggan capsules", the T1 of single medicine group was 21.3. The T1 of triple medicine group was 27. Conclusion The in vitro pharmacological experiment on inhibiting A/Brisban/10/2008 (H3N2) influenza virus A indicates that Niuhuang Qinggan capsules have inhibition and prevention effect for influenza virus A.