CAJ | 학술논문

目的建立中山地区常住人口CD4+T淋巴细胞计数生物参考区间。方法选取140例中山地区健康个体，分为15~19岁、20~29岁、30~39岁、40~49岁和50~59岁共5个年龄组。以Partec PAS流式细胞仪测定外周血+CD4 T淋巴细胞绝对数。结果同性别各年龄层之间以及不同性别之间的CD4+T细胞数差异均无统计学意义（P＞0.05）；合并各组个体结果，数据呈正态分布，以（x±1.96s）确定95%的参考区间为（804.7±387.3）个/+μL。结论成功建立本地区健康成人外周血CD4 T细胞计数的参考范围，为本地区艾滋病患者的临床分期诊断、抗病毒治疗效果提供参考依据。
목적건립중산지구상주인구CD4+T림파세포계수생물삼고구간。방법선취140례중산지구건강개체，분위15~19세、20~29세、30~39세、40~49세화50~59세공5개년령조。이Partec PAS류식세포의측정외주혈+CD4 T림파세포절대수。결과동성별각년령층지간이급불동성별지간적CD4+T세포수차이균무통계학의의（P＞0.05）；합병각조개체결과，수거정정태분포，이（x±1.96s）학정95%적삼고구간위（804.7±387.3）개/+μL。결론성공건립본지구건강성인외주혈CD4 T세포계수적삼고범위，위본지구애자병환자적림상분기진단、항병독치료효과제공삼고의거。
Objective To establish A biological reference interval of peripheral blood CD4+T lymphocytes for the permanent resident population in Zhongshan district. Methods 140 healthy individuals in Zhongshan district were selected and divided into 5 age goups including 15-19 years old,20-29 years old,30-39 years old,40-49 years old and 50-59 years old. The Partec PAS flow cytometer was used to measure the absolute number of peripheral blood CD4+T lymphocytes. Results The absolute numbers of CD4+T lymphocytes were not significantly different between different age groups of the same gender and different genders (P > 0.05); Combined results of all the individuals showed a normal distribution and the 95% reference interval determined by x±1.96s was (804.7±387.3)lymphocytes/μL. Conclusion The reference interval of peripheral blood CD4+T lymphocytes for the healthy population in this district was established successfully,which provides reference for the clinical staging diagnosis and antiviral treatment effect of AIDS patients in this district.