神经损伤与功能重建
神經損傷與功能重建
신경손상여공능중건
NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION
2014年
3期
203-204,245
,共3页
吴磊彬%邱昕%陈国华%魏丹%邵卫%李俐娟%张忠文%杨洁%吴潇%蔡薇娜%周晓香%熊晶
吳磊彬%邱昕%陳國華%魏丹%邵衛%李俐娟%張忠文%楊潔%吳瀟%蔡薇娜%週曉香%熊晶
오뢰빈%구흔%진국화%위단%소위%리리연%장충문%양길%오소%채미나%주효향%웅정
慢性束缚应激刺激%逍遥散%阿尔茨海默病%神经递质
慢性束縳應激刺激%逍遙散%阿爾茨海默病%神經遞質
만성속박응격자격%소요산%아이자해묵병%신경체질
chronic mild stress%Xiaoyaosan%Alzheimer's disease%neurotransmitter
目的：观察逍遥散对慢性应激下拟阿尔茨海默病（AD）小鼠记忆能力和血清单胺类神经递质的影响。方法：将50只小鼠随机分为空白对照组﹑D-半乳糖组﹑复合模型组﹑逍遥散组﹑艾司西酞普兰组，每组10只。空白对照组给予生理盐水皮下注射，D-半乳糖组﹑复合模型组﹑逍遥散组﹑艾司西酞普兰组均皮下注射D-半乳糖溶液42 d 造模。造模第29天开始，逍遥散组给予逍遥散灌胃，艾司西酞普兰组给予艾司西酞普兰灌胃，连续14 d；每日灌胃完成后，复合模型组、逍遥散组和艾司西酞普兰组均给予慢性束缚应激刺激，每天6 h，连续14 d。实验结束后，对各组进行避暗试验，并检测血清5-羟色胺（5-HT）和去甲肾上腺素（NE）含量。结果：避暗试验结果显示，与空白对照组和 D-半乳糖组比较，复合模型组潜伏期缩短，错误次数增多；与复合模型组比较，逍遥散组和艾司西酞普兰组潜伏期延长，错误次数减少，逍遥散组和艾司西酞普兰组相比无明显差异。与空白对照组和 D-半乳糖组比较，复合模型组5-HT 和 NE 均明显降低；与复合模型组比较，逍遥散组和艾司西酞普兰组5-HT 和 NE 均有升高，逍遥散组较艾司西酞普兰组升高更明显。结论：慢性束缚应激刺激联合 D-半乳糖注射可成功建立肝郁型 AD 病证结合模型；逍遥散能明显改善此模型的记忆能力，且提升血清5-HT 和 NE 的含量。
目的：觀察逍遙散對慢性應激下擬阿爾茨海默病（AD）小鼠記憶能力和血清單胺類神經遞質的影響。方法：將50隻小鼠隨機分為空白對照組﹑D-半乳糖組﹑複閤模型組﹑逍遙散組﹑艾司西酞普蘭組，每組10隻。空白對照組給予生理鹽水皮下註射，D-半乳糖組﹑複閤模型組﹑逍遙散組﹑艾司西酞普蘭組均皮下註射D-半乳糖溶液42 d 造模。造模第29天開始，逍遙散組給予逍遙散灌胃，艾司西酞普蘭組給予艾司西酞普蘭灌胃，連續14 d；每日灌胃完成後，複閤模型組、逍遙散組和艾司西酞普蘭組均給予慢性束縳應激刺激，每天6 h，連續14 d。實驗結束後，對各組進行避暗試驗，併檢測血清5-羥色胺（5-HT）和去甲腎上腺素（NE）含量。結果：避暗試驗結果顯示，與空白對照組和 D-半乳糖組比較，複閤模型組潛伏期縮短，錯誤次數增多；與複閤模型組比較，逍遙散組和艾司西酞普蘭組潛伏期延長，錯誤次數減少，逍遙散組和艾司西酞普蘭組相比無明顯差異。與空白對照組和 D-半乳糖組比較，複閤模型組5-HT 和 NE 均明顯降低；與複閤模型組比較，逍遙散組和艾司西酞普蘭組5-HT 和 NE 均有升高，逍遙散組較艾司西酞普蘭組升高更明顯。結論：慢性束縳應激刺激聯閤 D-半乳糖註射可成功建立肝鬱型 AD 病證結閤模型；逍遙散能明顯改善此模型的記憶能力，且提升血清5-HT 和 NE 的含量。
목적：관찰소요산대만성응격하의아이자해묵병（AD）소서기억능력화혈청단알류신경체질적영향。방법：장50지소서수궤분위공백대조조﹑D-반유당조﹑복합모형조﹑소요산조﹑애사서태보란조，매조10지。공백대조조급여생리염수피하주사，D-반유당조﹑복합모형조﹑소요산조﹑애사서태보란조균피하주사D-반유당용액42 d 조모。조모제29천개시，소요산조급여소요산관위，애사서태보란조급여애사서태보란관위，련속14 d；매일관위완성후，복합모형조、소요산조화애사서태보란조균급여만성속박응격자격，매천6 h，련속14 d。실험결속후，대각조진행피암시험，병검측혈청5-간색알（5-HT）화거갑신상선소（NE）함량。결과：피암시험결과현시，여공백대조조화 D-반유당조비교，복합모형조잠복기축단，착오차수증다；여복합모형조비교，소요산조화애사서태보란조잠복기연장，착오차수감소，소요산조화애사서태보란조상비무명현차이。여공백대조조화 D-반유당조비교，복합모형조5-HT 화 NE 균명현강저；여복합모형조비교，소요산조화애사서태보란조5-HT 화 NE 균유승고，소요산조교애사서태보란조승고경명현。결론：만성속박응격자격연합 D-반유당주사가성공건립간욱형 AD 병증결합모형；소요산능명현개선차모형적기억능력，차제승혈청5-HT 화 NE 적함량。
ObjectiveTo observe effects of Xiaoyaosan on the memory ability and neurotransmitters expression in Alzheimer's disease (AD) mouce model with chronic mild stress. Methods: Fifty mice were randomly divided into five groups: blank group, D-Gal group, compound group, Xiaoyaosan group and Escitalopram group, with 10 mice in each group. The blank group was injected with normal saline, and the others were injected with D-gal for 42 days. From the twenty-ninth day, the mice in the Xiaoyaosan group were intragastrically administrated with Xiaoyaosan, and in the Escitalopram group with escitalopram. After the intragastric administration, the mice in both the Xiaoyaosan and Escitalopram groups were subjected to chronic stress for 6 hours each day, lasting for 14 days. Then Dark evading test and NE and 5-HT levels in all the mice were evaluated. Results: Compared to the groups of blank and D-gal, the escape latency time in the compound group was shortened and error times were increased in the Dark evading test, while the 5-HT and NA levels were decreased markedly. Compared to the compound group, the escape latency time in the Xiaoyaosan and Escitalopram groups were prolonged and error times were reduced, while 5-HT and NA were increased. Conclusion: Chronic mild stress and D-gal combination can be used to establish AD mouce model with stagnation of liver-QI. Xiaoyaosan can improve memory function and increase the serum 5-HT and NE levels in AD mice.
