中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2013年
18期
1098-1101
,共4页
宋金莲%马中良%侯琳%马春红%王盛楠%吴琍
宋金蓮%馬中良%侯琳%馬春紅%王盛楠%吳琍
송금련%마중량%후림%마춘홍%왕성남%오리
WIF-1%基因表达%乳腺癌%启动子甲基化%TNM分期%临床病理特征
WIF-1%基因錶達%乳腺癌%啟動子甲基化%TNM分期%臨床病理特徵
WIF-1%기인표체%유선암%계동자갑기화%TNM분기%림상병리특정
WIF-1%gene expression%breast cancer%methylation%TNM stage
目的：研究WIF-1（Wnt inhibitory factor-1）mRNA在乳腺癌组织中的表达及其启动子区域甲基化情况，进一步探讨WIF-1基因甲基化与乳腺癌临床病理特征的关系。方法：收集2009年9月1日至2009年12月30日青岛大学附属医院乳腺外科手术切除新鲜组织标本69例，其中良性病变组织9例，乳腺癌及癌旁组织各30例，应用RT-PCR及甲基化特异性PCR（methyla-tion specific PCR，MSP）检测乳腺癌组织、相应癌旁组织和乳腺良性病变组织中WIF-1mRNA表达及其启动子甲基化情况。结果：癌组织中WIF-1基因表达率明显低于相应癌旁组织及乳腺良性病变组织，具有显著性差异（χ2=41.786，P<0.05）；与其他两组相比甲基化率在癌组织中明显升高（矫正χ2=16.484，P<0.05）；WIF-1基因表达下降与其异常甲基化存在明显关联（P=0.023）；WIF-1异常甲基化与乳腺癌发病年龄、肿瘤分级、组织分型和淋巴结转移无相关性（P>0.05）。结论：异常甲基化可能是乳腺癌WIF-1基因表达下降的重要原因，是乳腺癌发生、发展的重要机制。
目的：研究WIF-1（Wnt inhibitory factor-1）mRNA在乳腺癌組織中的錶達及其啟動子區域甲基化情況，進一步探討WIF-1基因甲基化與乳腺癌臨床病理特徵的關繫。方法：收集2009年9月1日至2009年12月30日青島大學附屬醫院乳腺外科手術切除新鮮組織標本69例，其中良性病變組織9例，乳腺癌及癌徬組織各30例，應用RT-PCR及甲基化特異性PCR（methyla-tion specific PCR，MSP）檢測乳腺癌組織、相應癌徬組織和乳腺良性病變組織中WIF-1mRNA錶達及其啟動子甲基化情況。結果：癌組織中WIF-1基因錶達率明顯低于相應癌徬組織及乳腺良性病變組織，具有顯著性差異（χ2=41.786，P<0.05）；與其他兩組相比甲基化率在癌組織中明顯升高（矯正χ2=16.484，P<0.05）；WIF-1基因錶達下降與其異常甲基化存在明顯關聯（P=0.023）；WIF-1異常甲基化與乳腺癌髮病年齡、腫瘤分級、組織分型和淋巴結轉移無相關性（P>0.05）。結論：異常甲基化可能是乳腺癌WIF-1基因錶達下降的重要原因，是乳腺癌髮生、髮展的重要機製。
목적：연구WIF-1（Wnt inhibitory factor-1）mRNA재유선암조직중적표체급기계동자구역갑기화정황，진일보탐토WIF-1기인갑기화여유선암림상병리특정적관계。방법：수집2009년9월1일지2009년12월30일청도대학부속의원유선외과수술절제신선조직표본69례，기중량성병변조직9례，유선암급암방조직각30례，응용RT-PCR급갑기화특이성PCR（methyla-tion specific PCR，MSP）검측유선암조직、상응암방조직화유선량성병변조직중WIF-1mRNA표체급기계동자갑기화정황。결과：암조직중WIF-1기인표체솔명현저우상응암방조직급유선량성병변조직，구유현저성차이（χ2=41.786，P<0.05）；여기타량조상비갑기화솔재암조직중명현승고（교정χ2=16.484，P<0.05）；WIF-1기인표체하강여기이상갑기화존재명현관련（P=0.023）；WIF-1이상갑기화여유선암발병년령、종류분급、조직분형화림파결전이무상관성（P>0.05）。결론：이상갑기화가능시유선암WIF-1기인표체하강적중요원인，시유선암발생、발전적중요궤제。
Objective:To investigate the mRNA expression of the WIF-1 gene and the methylation of its promoter in breast can-cer, and to determine the correlation between the epigenetic aberrant WIF-1 DNA methylation and the clinicopathological significance of WIF-1 in breast cancer . Methods:RT-PCR and sensitive methylation-specific-PCR (MSP) were used to detect WIF-1 mRNA ex-pression and the methylation of the WIF-1 promoter in 30 breast cancer samples as well as in tumor-adjacent tissue samples and 9 be-nign breast tissues. Results:The WIF-1 mRNA expression in 30 breast cancer samples significantly decreased compared with those of the other two groups. In addition, WIF-1 methylation was more frequent in breast-tumor tissues compared with those in tumor-free tis-sues. Meanwhile, WIF-1 mRNA expression in breast cancer tissues involved the abnormal methylation of its promoter. Clinicopatholog-ical correlation analysis showed that the abnormal methylation of the WIF-1 gene promoter was not associated with age, TNM stage, histotype, or lymph node metastasis. Conclusion:WIF-1 mRNA expression loss due to abnormal methylation may be a crucial factor in breast cancer development and can thus be used in the prognosis and progression of the disease.
