CAJ | 학술논문

目的：建立反相高效液相色谱法测定3-酰胺基-吲哚类衍生物及其有关物质含量。方法采用Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇乙腈水(431245),流速1.0 mL·min-1；检测波长224 nm；进样量20μL；柱温：室温。结果3-酰胺基-吲哚类衍生物与有关物质能达到较好分离,在0.04~0.16 mg·mL-1浓度范围内与峰面积线性关系良好,r=0.9997；方法重复性RSD为0.52%；检测限为2.65 ng。结论该方法准确可靠,灵敏度高,专属性好,可用于3-酰胺基-吲哚类衍生物及其有关物质含量测定。
목적：건립반상고효액상색보법측정3-선알기-신타류연생물급기유관물질함량。방법채용Diamonsil C18(250 mm×4.6 mm,5μm)색보주,류동상위갑순을정수(431245),류속1.0 mL·min-1；검측파장224 nm；진양량20μL；주온：실온。결과3-선알기-신타류연생물여유관물질능체도교호분리,재0.04~0.16 mg·mL-1농도범위내여봉면적선성관계량호,r=0.9997；방법중복성RSD위0.52%；검측한위2.65 ng。결론해방법준학가고,령민도고,전속성호,가용우3-선알기-신타류연생물급기유관물질함량측정。
Objective To establish a RP-HPLC method for the determination of 3-amide-indole derivative and its related substances. Methods Diamonsil C18(250 mmí4. 6 mm,5 μm) column was adopted. The mobile phase consisted of a mixture of methanol-acetonitrile-water(431245) at the flow rate of 1. 0 mL·min-1 . The wavelength for ultraviolet detection was 224 nm. The injection volume was 20 μL and the column temperature was room temperature. Results 3-amide-indole derivative and related substances could be well separated. The linearity of the 3-amide-indole derivative curve was well correlated (r=0. 999 7) within the range of 0. 04-0. 16 mg·mL-1. The RSD was 0. 52%with good repeatability. The detection limit was 2. 65 ng. Conclusion The method is accurate,reliable,sensitive and specific,which could be used for the determination of 3-amide-indolederivative and related substances.