CAJ | 학술논문

本文通过对原油的充分降解生成甲烷气体，可以将不易开采含有剩余油的油藏变为更容易开采的气藏。以大庆油田不同区块油井产出水为菌种来源，利用原油为微生物唯一碳源。45℃封闭条件下培养。培养2个月后，开始检测到甲烷气体，5克原油底物通过微生物作用，12个月累计产气量为81mL。根据菌群代谢功能，可以分为三类，分别为烃降解菌、乙酸盐菌和产甲烷菌，这三大类菌完成了由原油生成甲烷的过程。对筛选出的产甲烷古菌进行分类鉴定，鉴定结果分别为甲烷囊菌属（Methanocul eus）和嗜热甲烷鬃菌（Methanosaeta ther-mophila）。
본문통과대원유적충분강해생성갑완기체，가이장불역개채함유잉여유적유장변위경용역개채적기장。이대경유전불동구괴유정산출수위균충래원，이용원유위미생물유일탄원。45℃봉폐조건하배양。배양2개월후，개시검측도갑완기체，5극원유저물통과미생물작용，12개월루계산기량위81mL。근거균군대사공능，가이분위삼류，분별위경강해균、을산염균화산갑완균，저삼대류균완성료유원유생성갑완적과정。대사선출적산갑완고균진행분류감정，감정결과분별위갑완낭균속（Methanocul eus）화기열갑완종균（Methanosaeta ther-mophila）。
Thispaperattemptbytheful biodegradationofCrudeoilforproducemethanegas,canbedifficulttocontaintheremainingoilintoareservoir more easily evelopment gas reservoir. adding only one gram crude oil.In this study, bacteria resource was oil wel produced water at different blocks of DaqingOilfield,thesolemicrobialcarbonsourcewascrudeoil.Methanewasdetectedafter2monthsofcultureataclosedmediumunder45℃.Bymi-crobial metabolism, 81mL cumulative gas was obtained which was converted by 5g oil in 12 months.The flora can be divided into three categories ac-cording to the metabolic function, which include hydrocarbon degradation bacteria, acetic acid bacteria and methane- producing bacteria, and these threecategoriescompletedtheprocessofmethane- producingbycrudeoil.AndthescreenedMethanogenicArchaeawereidentifiedasMethanocul eus andMethanosaetathermophila.