CAJ | 학술논문

目的:探讨低相对分子质量姬松茸多糖(low molecular weight polysaccharide isolated from Agaricus blazei Murill,LMPAB)对树突状细胞C型植物血凝素1(Dectin-1),脾酪氨酸激酶(Sky)和胱天蛋白募集域蛋白9(CARD9)基因表达和T淋巴细胞增殖的影响.方法:体外培养ICR小鼠骨髓来源的树突状细胞;通过流式细胞术检测树突状细胞表面CD11c和CD86分子的表达;采用WST方法检测LMPAB增强树突状细胞对T淋巴细胞的增殖作用;应用实时定量PCR方法检测Dectin-1,Sky和CARD9 mRNA的表达水平.结果:树突状细胞表面CD11c表达98.6％,CD86表达96.97％;与正常组(4.84±0.21)比较,LMPAB +OVA组(6.59±0.19)可增强树突状细胞对T淋巴细胞的增殖作用(P＜0.05);与正常组Dectin-1 (6.13±0.27),Sky(0.36 ±0.11)和CARD9(1.85±0.20)比较,LMPAB+OVA组Dectin-1(8.25±0.19),Sky(1.63±0.16)和CARD9(4.40±0.19)明显升高,统计学具有显著意义(P＜0.05).结论:所培养的髓系细胞为树突状细胞;LMPAB可通过调控树突状细胞Dectin-1,Sky和CARD9 mRNA的表达增强树突状细胞的免疫效能.
목적:탐토저상대분자질량희송용다당(low molecular weight polysaccharide isolated from Agaricus blazei Murill,LMPAB)대수돌상세포C형식물혈응소1(Dectin-1),비락안산격매(Sky)화광천단백모집역단백9(CARD9)기인표체화T림파세포증식적영향.방법:체외배양ICR소서골수래원적수돌상세포;통과류식세포술검측수돌상세포표면CD11c화CD86분자적표체;채용WST방법검측LMPAB증강수돌상세포대T림파세포적증식작용;응용실시정량PCR방법검측Dectin-1,Sky화CARD9 mRNA적표체수평.결과:수돌상세포표면CD11c표체98.6％,CD86표체96.97％;여정상조(4.84±0.21)비교,LMPAB +OVA조(6.59±0.19)가증강수돌상세포대T림파세포적증식작용(P＜0.05);여정상조Dectin-1 (6.13±0.27),Sky(0.36 ±0.11)화CARD9(1.85±0.20)비교,LMPAB+OVA조Dectin-1(8.25±0.19),Sky(1.63±0.16)화CARD9(4.40±0.19)명현승고,통계학구유현저의의(P＜0.05).결론:소배양적수계세포위수돌상세포;LMPAB가통과조공수돌상세포Dectin-1,Sky화CARD9 mRNA적표체증강수돌상세포적면역효능.