CAJ | 학술논문

目的：观察Siah2（ seven in absentia2）及脯氨酰羟化酶3（prolyl hydroxylase 3，PHD3）在人非小细胞肺癌及癌旁组织中的表达，并探讨上述两者差异表达在非小细胞肺癌发病过程中的临床意义。方法应用RT-PCR检测非小细胞肺癌患者89例肿瘤及癌旁正常肺组织标本Siah2、PHD3 mRNA表达情况， Western blot 检测上述指标的蛋白质表达水平，并分析其与肺癌临床特征的关系。结果非小细胞肺癌患者肿瘤组织中Siah2蛋白及mRNA表达量均明显高于癌旁组织（ P ＜0殮．05），PHD3蛋白的表达在肿瘤组织中低于癌旁组织（ P ＜0．05）， PHD3 mRNA在肿瘤组织中的表达高于癌旁组织，Siah2蛋白与PHD3蛋白表达呈负相关（ r ＝-0．735， P ＜0．05），Siah2蛋白与Siah2 mRNA表达呈正相关（ r ＝0．821， P ＜0．05）。结论 Siah2可能通过调控PHD3表达水平在非小细胞肺癌发病中发挥作用。
목적：관찰Siah2（ seven in absentia2）급포안선간화매3（prolyl hydroxylase 3，PHD3）재인비소세포폐암급암방조직중적표체，병탐토상술량자차이표체재비소세포폐암발병과정중적림상의의。방법응용RT-PCR검측비소세포폐암환자89례종류급암방정상폐조직표본Siah2、PHD3 mRNA표체정황， Western blot 검측상술지표적단백질표체수평，병분석기여폐암림상특정적관계。결과비소세포폐암환자종류조직중Siah2단백급mRNA표체량균명현고우암방조직（ P ＜0렴．05），PHD3단백적표체재종류조직중저우암방조직（ P ＜0．05）， PHD3 mRNA재종류조직중적표체고우암방조직，Siah2단백여PHD3단백표체정부상관（ r ＝-0．735， P ＜0．05），Siah2단백여Siah2 mRNA표체정정상관（ r ＝0．821， P ＜0．05）。결론 Siah2가능통과조공PHD3표체수평재비소세포폐암발병중발휘작용。
Objective To investigate the expression of Siah2(seven in absentia2) and prolyl hydroxylase 3(PHD3) in non-small cell lung cancer ( NSCLC) ,and to elucidate their clinical significance .Methods The mRNA expression levels of Siah2 and PHD3 were detected by RT-PCR in 89 cases of NSCLC and adjacent normal lung tissues , and their protein expression levels were detected by Western Blot .Results The expression levels of Siah 2 mRNA and protein in tumor tissues were significantly higher than those in adjacent tissues ( P <0.05).The expression levels of PHD3 mRNA in tumor tissues were obviously higher than those in adjacent tissues , however , the expression levels of PHD 3 protein in tumor tissues were significantly lower than those in adjacent tissues ( P <0.05).The expression of Siah2 protein was negatively correlated to that of PHD3 protein( r =-0.735, P <0.05),however,the expression of Siah2 protein was positively related with that of Siah 2 mRNA( r =0.821, P <0.05).Conclusion Siah2 may play a role in pathogenesis of NSCLC by regulating PHD 3 expression levels.