CAJ | 학술논문

以羟基乙酸(GA)和1,10-邻菲咯啉(phen)作为配体,合成了一种新型的三元单核钴(II)配合物,Co (GA)2(phen)·2H2O。通过傅里叶红外光谱和元素分析法对该配合物的结构进行了表征,并利用X射线单晶衍射测定了其晶体结构。晶体分析结果表明配合物属单斜晶系,C2/c空间群,晶胞参数：a=8．122(3)nm,b=24．214(7)nm,c=9．085(3)nm,α=105．290(14)°,β=109．843(5)°,γ=90．00°,晶胞体积：V=680．7(9)nm3,晶胞内结构分子数Z=4,最终的偏差因子：R1,wR2分别为0．0463,0．1294[I>2σ(I)]。中心钴离子分别与两个羟基乙酸及一个邻菲咯啉配体配位,形成一个畸变的八面体配位几何构型。采用电子吸收光谱、荧光光谱、粘度等分析方法研究了该配合物与DNA的相互作用,结果表明该配合物的通过经典的嵌插方式与DNA结合,二者的结合常数Kb为3．8×104 L·mol-1。琼脂糖凝胶电泳实验进一步表明,在过氧化氢(H2O2)的存在条件下,配合物能对质粒体超螺旋DNA产生切割作用,具有化学核酸酶活性。
이간기을산(GA)화1,10-린비각람(phen)작위배체,합성료일충신형적삼원단핵고(II)배합물,Co (GA)2(phen)·2H2O。통과부리협홍외광보화원소분석법대해배합물적결구진행료표정,병이용X사선단정연사측정료기정체결구。정체분석결과표명배합물속단사정계,C2/c공간군,정포삼수：a=8．122(3)nm,b=24．214(7)nm,c=9．085(3)nm,α=105．290(14)°,β=109．843(5)°,γ=90．00°,정포체적：V=680．7(9)nm3,정포내결구분자수Z=4,최종적편차인자：R1,wR2분별위0．0463,0．1294[I>2σ(I)]。중심고리자분별여량개간기을산급일개린비각람배체배위,형성일개기변적팔면체배위궤하구형。채용전자흡수광보、형광광보、점도등분석방법연구료해배합물여DNA적상호작용,결과표명해배합물적통과경전적감삽방식여DNA결합,이자적결합상수Kb위3．8×104 L·mol-1。경지당응효전영실험진일보표명,재과양화경(H2O2)적존재조건하,배합물능대질립체초라선DNA산생절할작용,구유화학핵산매활성。
A novel mononuclear cobalt(II)complex containing mixed-ligands of glycollic acid(GA)and 1,10-phenathroline(phen), Co(GA)2(phen)·2H2O was synthesized and its structure was characterized by IR,element analysis and X-ray diffraction meth-ods. Crystal data suggested that the complex crystallizes in monoclinic system,space group C2/c with the dimension:a=8. 122(3) nm,b=24. 214(7)nm,c=9. 085(3)nm,α=105. 290(14)°,β=109. 843(5)°,γ=90. 00°,V=680. 7(9)nm3,Z=4,R1=0. 0463, wR2=0. 1294[I>2σ(I)]. The central cobalt(II)ion was coordinated with two GA and one phen ligands,displaying adistorted octa-hedron coordination geometry. The interaction of the complex with DNA was investigated by electronic absorption spectroscopy,fluo-rescence spectroscopy and viscosity methods. All the results revealed that the cobalt( II) complex could interact with DNA via a typi-cal intercalation mode with the binding constant of 3. 8 × 104 L·mol-1 . Agarose gel electrophoresis assays further showed that Co (GA)2(phen)·2H2O could effectively cleavage the supercoiled plasmid pBR322 DNA in the presence of H2O2,presenting an arti-ficial nuclease activity.