CAJ | 학술논문

目的：观察电针溃疡性结肠炎（ulcerative colitis, UC）大鼠大肠经经合穴“曲池”、下合穴“上巨虚”后对核因子-κB((nuclearfactorκB，NF-κB)、高迁移率族蛋白1(high mobility group box 1， HMGB1)表达的影响。方法以2-4-6三硝基苯磺酸（TNBS）/乙醇溶液灌肠诱导建立SD大鼠UC模型，曲池组与上巨虚组造模成功后分别电针曲池穴和上巨虚穴，连续治疗10 d，第11天处死所有大鼠，肉眼观察大鼠结肠溃疡评分,提取结肠组织检测其NF-κB、HMGB1的表达。结果造模成功后，模型组大鼠结肠肉眼溃疡评分、结肠组织NF-κB、HMGB1表达均明显增高（P<0.01）；电针治疗后，上巨虚组降低溃疡评分（P<0.01），曲池组、上巨虚组均能降低NF-κB、HMGB1在结肠中的表达（P<0.01）；与曲池组相比，上巨虚组溃疡评分及NF-κB、HMGB1表达均明显降低（P<0.01）。结论电针对UC的治疗作用可能是通过调节NF-κB、HMGB1等来实现的；电针上巨虚的治疗作用优于曲池，说明“上巨虚”存在相对特异性，部分证实“合治内府”中的“合”主要应指下合穴，其具体内涵仍需进一步研究。
목적：관찰전침궤양성결장염（ulcerative colitis, UC）대서대장경경합혈“곡지”、하합혈“상거허”후대핵인자-κB((nuclearfactorκB，NF-κB)、고천이솔족단백1(high mobility group box 1， HMGB1)표체적영향。방법이2-4-6삼초기분광산（TNBS）/을순용액관장유도건립SD대서UC모형，곡지조여상거허조조모성공후분별전침곡지혈화상거허혈，련속치료10 d，제11천처사소유대서，육안관찰대서결장궤양평분,제취결장조직검측기NF-κB、HMGB1적표체。결과조모성공후，모형조대서결장육안궤양평분、결장조직NF-κB、HMGB1표체균명현증고（P<0.01）；전침치료후，상거허조강저궤양평분（P<0.01），곡지조、상거허조균능강저NF-κB、HMGB1재결장중적표체（P<0.01）；여곡지조상비，상거허조궤양평분급NF-κB、HMGB1표체균명현강저（P<0.01）。결론전침대UC적치료작용가능시통과조절NF-κB、HMGB1등래실현적；전침상거허적치료작용우우곡지，설명“상거허”존재상대특이성，부분증실“합치내부”중적“합”주요응지하합혈，기구체내함잉수진일보연구。
Objective To observe the expression level of high m obility group box 1 (HMGB1), nuclearfactorκB (N F-κB) in the ulcerative colitis(UC ) rats and study related m echanism to further understand the m eaning of"He-sea points" in "curing viscera diseases by He-sea points" after electro-acupuncturing "Quchi" and "Shangjuxu" acupoints. Methods The UC rats m odels were given trinitrobenzene sulphoneacid (TN BS)/ethanol enem a, then "Quchi" group and "Shangjuxu" group respec-tively received electro-acupuncture at"Quchi" and "Shangjuxu" acupoints for 10 consecutive days. 24h after the last treatm ent, we dissected all rats and collected colon tissue to observe ulcer score and the expres-sion level of HMGB1 and N F-κB. R esults ① Compared with control group, thulcer score,HMGB1 and NF-κB signifcantly increased (P<0.01) in m odel group.② Compared with model group,"Quchi" group and "Shangjuxu" group made obvious decrease in the ulcer score,HMGB1 and NF-κB (P<0.01).③ Com-pared with "Quchi" group, the ulcer score,HMGB1 and NF-κB decreased significantly(P<0.01). Conclusion ① Both of the large intestinal meridian's lower He-sea point "Shangjuxu" and upper He-sea point"Quchi" can improve the UC through decreasing the ulcer score , HMGB1 and N F-κB of the ulcera-tive colitis. ② The treatment effect of "Shangjuxu" on the UC is better than "Quchi", it shows that"Shangjuxu" possess relative specificity.③ Our results im partially suggest that"He points" in "curing vis-cera diseases by He points" mainly implicates the lower He point, and the specific mechanism still needs further research.