CAJ | 학술논문

确保合并血浆检测结果的判定准确可靠，能够有效保证合并血浆的病毒安全性，对合并血浆乙型肝炎病毒表面抗原、丙型肝炎病毒抗体、人类免疫缺陷病毒抗体检测时不同厂家检测试剂的临界值进行确定。(1)使用双抗体夹心酶联免疫法对合并血浆HBsAg和HIV-1/HIV-2抗体的检测临界值进行确定。(2)使用酶联免疫法检测合并血浆HCV抗体的临界值进行确定。经检测和计算，两个厂家检测试剂的检测临界值系数分别为乙型肝炎病毒表面抗原23．398%和26．845%、丙型肝炎病毒抗体9．012%和16．481%、人类免疫缺陷病毒抗体20．025%和23．424%。
학보합병혈장검측결과적판정준학가고，능구유효보증합병혈장적병독안전성，대합병혈장을형간염병독표면항원、병형간염병독항체、인류면역결함병독항체검측시불동엄가검측시제적림계치진행학정。(1)사용쌍항체협심매련면역법대합병혈장HBsAg화HIV-1/HIV-2항체적검측림계치진행학정。(2)사용매련면역법검측합병혈장HCV항체적림계치진행학정。경검측화계산，량개엄가검측시제적검측림계치계수분별위을형간염병독표면항원23．398%화26．845%、병형간염병독항체9．012%화16．481%、인류면역결함병독항체20．025%화23．424%。
Assurance the result exact and reliable for pool plasma analysis for decrease the risk of virus infection. Using test kits from different vendor to determine of a cut-off limit for virus mark detecting in pool plasma samples. The double-antibody sandwich ELISA technique was used for HBsAg and anti-HIV-1/HIV-2 test. The ELISA technique was used for anti-HCV test. The HBsAg cut-off limits for virus mark detecting of two vendor kits are 23. 398%and 26. 845%, The anti-HIV-1/HIV-2 cut-off limits are 20. 025%and 23. 424%,The anti-HCV cut-off limits are 9. 012%and 16. 481%. Cut-off limits of two vendor’ s test kits were deter-mined,and the limits can be used to judge detecting results of pool plasma samples.