CAJ | 학술논문

天然多酚化合物是蛋白质淀粉样纤维化相关疾病的潜在治疗药物。本文采用溶菌酶和表没食子儿茶素( EGC)研究了多酚化合物对蛋白质淀粉样纤维的作用。结果表明,EGC能够抑制溶菌酶的淀粉样纤维化,并能够破坏成熟的纤维结构,使纤维的淀粉样特性降低。巯基化合物二巯基苏糖醇( DTT)能够部分抑制EGC破坏纤维的作用,表明EGC可能通过与巯基结合而对多肽链进行共价修饰,从而改变了淀粉样纤维的自组装结构。根据上述结果,我们认为,多酚化合物形成醌类中间体并对多肽链的自由巯基进行修饰,是其抑制蛋白质淀粉样纤维化的主要途径。
천연다분화합물시단백질정분양섬유화상관질병적잠재치료약물。본문채용용균매화표몰식자인다소( EGC)연구료다분화합물대단백질정분양섬유적작용。결과표명,EGC능구억제용균매적정분양섬유화,병능구파배성숙적섬유결구,사섬유적정분양특성강저。구기화합물이구기소당순( DTT)능구부분억제EGC파배섬유적작용,표명EGC가능통과여구기결합이대다태련진행공개수식,종이개변료정분양섬유적자조장결구。근거상술결과,아문인위,다분화합물형성곤류중간체병대다태련적자유구기진행수식,시기억제단백질정분양섬유화적주요도경。
Natural polyphenols had been recognized as medicinal candidates for treating amyloid diseases. In the present study,the inhibitory effect of epigallocatechin( EGC) on amyloid formation was investigated in vitro by using lysozyme as a model protein. The results demonstrated that EGC inhibited efficiently lysozyme fibril formation and transformed the mature fibrils into non-amyloid as-semblies. Thiol compound dithiothreitol( DTT) abolished partly the fibril-destabilizing role of EGC,indicating that EGC probably a-lerted amyloid fibrillar structure through covalently binding to the peptide chains. It was suggested that formation of quinone interme-diates and subsequently modification of free thiol groups in peptide chains were the main events in the inhibition of lysozyme amy-loid fibrillation by polyphenols.