CAJ | 학술논문

本文利用 Nafion-氧化石墨烯复合物和硫堇构建了葡萄糖生物传感器。首先将氧化石墨烯分散在0．2%Nafion溶液中制得 Nafion-氧化石墨烯的复合物，并将其固定在玻碳电极表面，通过静电吸附将带正电荷的硫堇吸附到 Nafion-氧化石墨烯复合膜修饰的玻碳电极表面，然后利用硫堇的氨基和醛基化葡萄糖氧化酶的醛基共价键合作用将葡萄糖氧化酶固定到电极表面。实验表明该传感器响应快、灵敏度高、稳定性好。传感器的灵敏度为7．68μAcm-2( mmol·L-1)-1。
본문이용 Nafion-양화석묵희복합물화류근구건료포도당생물전감기。수선장양화석묵희분산재0．2%Nafion용액중제득 Nafion-양화석묵희적복합물，병장기고정재파탄전겁표면，통과정전흡부장대정전하적류근흡부도 Nafion-양화석묵희복합막수식적파탄전겁표면，연후이용류근적안기화철기화포도당양화매적철기공개건합작용장포도당양화매고정도전겁표면。실험표명해전감기향응쾌、령민도고、은정성호。전감기적령민도위7．68μAcm-2( mmol·L-1)-1。
A glucose biosensor was constructed based on the nafion,graphene oxide and thionine. First,the graphene oxide composite was prepared by dispersing graphene oxide in nafion solution,and dropped onto the surface of glass carbon electrode to form a stable film. Secondly,the obtained electrode was immersed in thionine solution to absorb the thionine molecules. The thionine was utilized to fabricate glucose biosensor with periodate oxidized glucose oxidase through covalent attachment. The biosensor exhibited fast re-sponse,high sensitivity and good stability. The sensitivity of the biosensor was 7. 68 μAcm-2(mmol·L-1)-1.