CAJ | 학술논문

采用化学处理方法在石墨烯表面引入羟基和羧基等活性基团,并将其沉积在玻碳电极表面制作成氧化石墨烯修饰电极,然后运用循环伏安法研究了氧氟沙星在该修饰电极上的电化学行为。结果表明,该修饰电极对氧氟沙星的电化学氧化表现出较强的电催化作用。在最佳实验条件下,采用灵敏度较高的示差脉冲伏法测定了溶液中的氧氟沙星浓度,其浓度在7．5×10-7至1．0×10-4mol·L-1范围内与氧化峰电流呈良好的线性关系,相关相关系数r为0．9931,检出限为2．2×10-7mol·L-1(S/N=3)。在1h之内对含2．5×10-5mol·L-1氧氟沙星的溶液进行11次平行测定,相对标准偏差为3．6%,表明修饰电极的重现性和稳定性好。将该修饰电极用于氧氟沙星滴眼液中氧氟沙星含量的测定,平均回收率为98．2%。与其他方法相比,该方法简便、快速,灵敏度高,选择性好,可用于药物中氧氟沙星的分析。
채용화학처리방법재석묵희표면인입간기화최기등활성기단,병장기침적재파탄전겁표면제작성양화석묵희수식전겁,연후운용순배복안법연구료양불사성재해수식전겁상적전화학행위。결과표명,해수식전겁대양불사성적전화학양화표현출교강적전최화작용。재최가실험조건하,채용령민도교고적시차맥충복법측정료용액중적양불사성농도,기농도재7．5×10-7지1．0×10-4mol·L-1범위내여양화봉전류정량호적선성관계,상관상관계수r위0．9931,검출한위2．2×10-7mol·L-1(S/N=3)。재1h지내대함2．5×10-5mol·L-1양불사성적용액진행11차평행측정,상대표준편차위3．6%,표명수식전겁적중현성화은정성호。장해수식전겁용우양불사성적안액중양불사성함량적측정,평균회수솔위98．2%。여기타방법상비,해방법간편、쾌속,령민도고,선택성호,가용우약물중양불사성적분석。
In present paper,graphene was functionalized with hydroxyl and carboxyl groups via chemical method and then deposited on the surface of glassy carbon electrode for preparing modified electrode. The electrochemical performance of the graphene modified electrode was also investigated as a new sensor for the detection of ofloxacin. It is found that the modified electrode exhibits an ex-cellent electrocatalytic activity toward the oxidation of ofloxacin. Based on this phenomenon,a simple,convenient and sensitive elec-trochemical method for determination of ofloxacin was developed. Under the optimized experimental conditions,the differential pulse stripping voltammetry( DPV) method was applied as a sensitive method for the quantitative detection of ofloxacin. A linear concen-tration range between 7. 5×10-7and 1. 0×10-4 mol·L-1 with a detection limit of 2. 2×10-7 mol·L-1(S/N=3)for ofloxacin is obtained from DPV measurements. The results of voltammetric determinations show a very good reproducibility,and the relative standard devi-ation(RSD)was 3. 6%for the slope of the calibration curve based on the 11 measurements of 2. 5×10-5mol·L-1 ofloxacin during one hour. The proposed method was applied successfully for determination of ofloxacin in drug samples with average recovery of 98. 2%. Moreover, compared to the other methods, the present one has the advantages of simplicity, high sensitivity and good selectivity. Therefore,it is a promising method for analysis ofloxacin in drug samples.