CAJ | 학술논문

利用随机扩增多态性DNA（ RAPD）技术对云南南湖和响水河两个鲫（ Carassius auratus）群体进行了遗传多样性分析，在50条随机引物中筛选出10条多态性较好的引物，通过PCR扩增，10条引物在南湖鲫群体中共检测到82个位点，其中多态性位点69个，平均多态性位点比率为83.56％，平均杂合度为0.2896， Shannon表型多样性指数为0.4320，群体内遗传相似率为0.6437；响水河鲫群体共检测到70个位点，其中多态性位点58个，平均多态位点比率为82.17％，平均杂合度为0.3167， Shannon表型多样性指数为0.4794，群体内遗传相似率为0.7318；两群体间的遗传相似率为0.9009，遗传距离为0.1043。结果表明南湖和响水河鲫群体都具有较高的遗传多样性，但南湖鲫群体稍高于响水河群体，而两群体间的遗传分化并不是很大。
이용수궤확증다태성DNA（ RAPD）기술대운남남호화향수하량개즉（ Carassius auratus）군체진행료유전다양성분석，재50조수궤인물중사선출10조다태성교호적인물，통과PCR확증，10조인물재남호즉군체중공검측도82개위점，기중다태성위점69개，평균다태성위점비솔위83.56％，평균잡합도위0.2896， Shannon표형다양성지수위0.4320，군체내유전상사솔위0.6437；향수하즉군체공검측도70개위점，기중다태성위점58개，평균다태위점비솔위82.17％，평균잡합도위0.3167， Shannon표형다양성지수위0.4794，군체내유전상사솔위0.7318；량군체간적유전상사솔위0.9009，유전거리위0.1043。결과표명남호화향수하즉군체도구유교고적유전다양성，단남호즉군체초고우향수하군체，이량군체간적유전분화병불시흔대。
Random amplified polymorphism DNA ( RAPD) was used to determine the genetic diversity of the two Carassius auratus populations in the South Lake and the Xiangshuihe reservoir at Mengzi , Yunnan province , China.Of 50 primers detected by PCR methods , 10 primers denoted highly reproductive and were used for genetic studies .Total 82 RAPD loci were detected in South Lake population , including , 69 polymorphic loci , and the percentage of polymorphism was 83.65%, the average heterozygosity was 0.289 6 , Shannon′s index of phenotypic was 0.432 0 , the genetic similarity ra-tio within the groups was 0.643 7; Total 70 loci were tested in Xiangshuihe population , including , 58 polymorphic loci , and the percentage of polymorphism was 82.17%, the average heterozygosity was 0.316 7 , the Shannon′s index of pheno-typic was 0.4794 , the genetic similarity ratio within the groups was 0.731 8; the genetic similarity ratio between the two groups was 0.900 9 , the genetic distance was 0.104 3 .The results showed that there was a high genetic diversity in the two C.auratus populations through that of the South Lake′s was even higher , and the genetic differentiation between the two populations was little .