CAJ | 학술논문

以5个不同品种的蝴蝶兰为材料,分别于叶面喷施不同浓度(0,0.2,0.4,0.6,0.8 mmol/L)的水杨酸(salicylic acid,SA)溶液,将其置于人工智能温室昼夜温度11℃/6℃的生长箱内低温胁迫处理3 d 和常温(27℃/22℃)恢复生长7 d,研究蝴蝶兰叶片抗氧化系统和光合系统的生理特征变化.结果表明：外源水杨酸对低温胁迫下蝴蝶兰叶片的伤害具有缓解效应,以0.4~0.6 mmol/L 的处理效果最佳,提高了蝴蝶兰叶片中的叶绿素、抗坏血酸(ascorbic acid,AsA)和谷胱甘肽(glutathione,GSH)质量分数以及 Fv/Fm 和超氧化物歧化酶(superoxide dismutase,SOD)活性,同时降低了抗坏血酸过氧化物酶(ascorbate peroxidase,APX)活性和可溶性糖质量分数.低温胁迫解除后,水杨酸处理能促进叶绿素质量分数的上升和 Fv/Fm 的恢复；提高了 SOD 酶活性和 GSH 的积累；降低了 APX 的活性以及 AsA 质量分数,促进细胞内光合系统和抗氧化系统的恢复.比较不同耐冷性的蝴蝶兰品种表明,耐冷性强的“婚宴”对水杨酸处理更为敏感,效果更为显著.
이5개불동품충적호접란위재료,분별우협면분시불동농도(0,0.2,0.4,0.6,0.8 mmol/L)적수양산(salicylic acid,SA)용액,장기치우인공지능온실주야온도11℃/6℃적생장상내저온협박처리3 d 화상온(27℃/22℃)회복생장7 d,연구호접란협편항양화계통화광합계통적생리특정변화.결과표명：외원수양산대저온협박하호접란협편적상해구유완해효응,이0.4~0.6 mmol/L 적처리효과최가,제고료호접란협편중적협록소、항배혈산(ascorbic acid,AsA)화곡광감태(glutathione,GSH)질량분수이급 Fv/Fm 화초양화물기화매(superoxide dismutase,SOD)활성,동시강저료항배혈산과양화물매(ascorbate peroxidase,APX)활성화가용성당질량분수.저온협박해제후,수양산처리능촉진협록소질량분수적상승화 Fv/Fm 적회복；제고료 SOD 매활성화 GSH 적적루；강저료 APX 적활성이급 AsA 질량분수,촉진세포내광합계통화항양화계통적회복.비교불동내랭성적호접란품충표명,내랭성강적“혼연”대수양산처리경위민감,효과경위현저.
Summary Phalaenopsis spp.is one of the most important orchids,which has a great economic value as potted plants in China.However,growth disorders like damage to membranes,generation of reactive oxygen species (ROS),protein denaturation,and accumulation of toxic compounds due to low temperature stress have become one of the key constraints for Phalaenopsis consumption during winter season in north subtropical area of China.There are a large number of reports about using salicylic acid (SA) to alleviate the negative effects of chilling stress on plants.However there is rare report about the changes of antioxidant systems under chilling stress with exogenous plant regulators application such as SA in Phalaenopsis . <br> The objectives of this study are to investigate the alleviating effects of exogenous SA application on Phalaenopsis under low temperature stress and in recovery growth,to determine the changes of antioxidative enzyme activities and key indicators of photosynthetic system. <br> Five different cultivars of Phalaenopsis (“Hunyan”“Dalajiao”“Tianjiao”“Tianxianggongzhu” and “V31”) were foliar sprayed with a series of SA concentrations (0,0.2,0.4,0.6,0.8 mmol/L).After foliar application,the plants were transferred to a growth chamber treated at low temperature(LT)of 1 1 ℃/6 ℃(day/night)for 3 days and then to recover growth at regular temperature 27 ℃ /22 ℃ (day/night)for 7 days.The changes of antioxidant system and photosynthetic system in Phalaenopsis leaves were tested. <br> The results showed that the foliar spray of SA could obviously alleviate the negative effects of LT stress.In this study,the best concentration of SA was from 0.4 to 0.6 mmol/L.Appropriate concentrations of SA could significantly increase the chlorophyll content,Fv/Fm,superoxide dismutase (SOD) activity,ascorbic acid (AsA) and glutathione (GSH) contents and improve the chilling tolerance of Phalaenopsis .The activity of ascorbate peroxidase(APX) and soluble sugar content were decreased in most of LT stress treatments.After LT stress, exogenous SA could promote the plant growth.After 7 days recovery growth,application of 0.6 mmol/L SA increased SOD and APX activities,chlorophyll content and Fv/Fm,and decreased AsA and GSH contents.The treatment enhanced the photosynthetic and antioxidant capacity of the plants. <br> These results indicated that the application of appropriate concentration of SA can improve the LT tolerance of Phalaenopsis .Compared the different varieties of Phalaenopsis ,it is concluded that the variety “Huanyan” is of strong LT tolerance and more sensitive and effective to SA application.