CAJ | 학술논문

采用细胞病变效应（CPE）观察和活细胞染色法（MTS），研究了银黄等几种天然植物药物在细胞培养模型（体外培养的锦鲤鳍条细胞）上抑制锦鲤疱疹病毒（ KHV）复制的效果。药物安全浓度试验结果表明，银黄、黄芪多糖、板蓝根、炎琥宁以及穿心莲对锦鲤鳍条组织细胞的安全浓度分别为15.63、2.5、31.25、31.25 mg/mL和6.25 mg/mL。在先给药后感染试验组，银黄、黄芪多糖、板蓝根和炎琥宁的浓度分别为安全浓度时，对KHV有明显的抑制效果，治疗指数分别为22.88±3.26、21.07±4.86、24.76±3.24和18.20±2.78；穿心莲抑制病毒复制的效果不明显。在先感染后给药试验组，银黄、板蓝根和炎琥宁浓度分别在安全浓度时，对KHV有显著的抑制作用，治疗指数分别为33.21±7.71、21.99±3.12和15.91±3.23。使用天然植物药物处理病毒后再感染细胞，仅观察到板蓝根在浓度为31.25 mg/mL体外对KHV有较弱的直接杀灭作用，治疗指数为5.7±1.62，而银黄、黄芪多糖、穿心莲和炎琥宁没有直接杀灭KHV的效果。
채용세포병변효응（CPE）관찰화활세포염색법（MTS），연구료은황등궤충천연식물약물재세포배양모형（체외배양적금리기조세포）상억제금리포진병독（ KHV）복제적효과。약물안전농도시험결과표명，은황、황기다당、판람근、염호저이급천심련대금리기조조직세포적안전농도분별위15.63、2.5、31.25、31.25 mg/mL화6.25 mg/mL。재선급약후감염시험조，은황、황기다당、판람근화염호저적농도분별위안전농도시，대KHV유명현적억제효과，치료지수분별위22.88±3.26、21.07±4.86、24.76±3.24화18.20±2.78；천심련억제병독복제적효과불명현。재선감염후급약시험조，은황、판람근화염호저농도분별재안전농도시，대KHV유현저적억제작용，치료지수분별위33.21±7.71、21.99±3.12화15.91±3.23。사용천연식물약물처리병독후재감염세포，부관찰도판람근재농도위31.25 mg/mL체외대KHV유교약적직접살멸작용，치료지수위5.7±1.62，이은황、황기다당、천심련화염호저몰유직접살멸KHV적효과。
By using the observation of cytopathic effect ( CPE) and the staining assay of live cells with MTS , herbal drugs were screened and evaluated for the inhibition of replication of koi herpesvirus ( KHV) in Koi-Fin cell line.The safety tests of drugs to cells showed that Yinhuang , Astragalus polysaccharide, Radix Isatidis, Herbal Andrographitis and An-drographolide were 15.63 mg/mL, 2.5 mg/mL, 31.25 mg/mL, 31.25 mg/mL and 6.25 mg/mL, respectively.In the tested group of drugs added prior to infection , while the concentrations of Yinhuang , Astragalus polysaccharide , R.Isatidis and Herbal Andrographitis were 15.63 mg/mL, 2.5 mg/mL, 31.25 mg/mL and 6.25 mg/mL, significant in-hibition of KHV replication were observed , the treatment index were 22.88 ±3.26 , 21.07 ±4.86 , 24.76 ±3.24 and 18.20 ±2.78 , respectively , but the Andrographolide is of no apparent inhibition.In the tested group that the infection was prior to drug addition, at the concentrations of Yinhuang , R.Isatidis and Herbal Andrographitis of 15.63 mg/mL, 31.25 mg/mL and 6.25 mg/mL, the significant effects in inhibition of KHV replication were achieved , the treatment index were 33.21 ±7.71 , 21.99 ±3.12 and 15.91 ±3.23 , respectively , while the Astragalus polysaccharide and Andrographolide had no apparent effects.In the tested group of co -incubation of virus and drugs before infection , at the concentrations of R.Isatidis of 31.25 mg/mL, low efficacy was observed in inhibiting KHV replication , the effect of treatment index were 5.7 ±1.62 , while the other drugs had no effects.