CAJ | 학술논문

探讨了大鲵（ Andrias davidianus）胚胎发育过程中受精率的评判标准和计算受精率的最佳时间。对大鲵胚胎发育观察表明：发育到二细胞的胚胎均能发育到神经胚期，因此可以将二细胞胚胎期作为受精的标志；孵化温度在17～20℃时，温度对大鲵的孵化率没有影响；胚胎发育经过24 h后，有90％～95％的受精卵可发育到二细胞期。因此可以以受精后24 h的二细胞分裂数作为计算大鲵胚胎受精率的基础数据。研究结果有利于对大鲵人工繁育效果提前判断和对孵化条件及时调控处理。
탐토료대예（ Andrias davidianus）배태발육과정중수정솔적평판표준화계산수정솔적최가시간。대대예배태발육관찰표명：발육도이세포적배태균능발육도신경배기，인차가이장이세포배태기작위수정적표지；부화온도재17～20℃시，온도대대예적부화솔몰유영향；배태발육경과24 h후，유90％～95％적수정란가발육도이세포기。인차가이이수정후24 h적이세포분렬수작위계산대예배태수정솔적기출수거。연구결과유리우대대예인공번육효과제전판단화대부화조건급시조공처리。
The relational fertilization tests of Chinese Andrias davidianus were carried out in order to analyze and evaluate the artificial insemination in early stage .During continuous observation of embryo development at early stages , the results proved that embryo at the two cell stage had the ability to develop dynamically to gastrula stage and neurula stage .Compar-ative analysis the time that fertility rate reached 90%and 95%revealed that 24 h after fertilized can be used as the optimal time.Combined the results , the embryo development at two cell stage and 24 h post-fertilization can serve as the judgment standard of fertility rate .The results served the important guidance for captive breeding in Chinese giant salamander .