淡水渔业
淡水漁業
담수어업
FRESHWATER FISHERIES
2014年
3期
80-84
,共5页
维生素D3%黄颡鱼(Pelteobagrus fulvidraco)%抗氧化功能%补体C3%补体C4
維生素D3%黃顙魚(Pelteobagrus fulvidraco)%抗氧化功能%補體C3%補體C4
유생소D3%황상어(Pelteobagrus fulvidraco)%항양화공능%보체C3%보체C4
vitamin D3%Pelteobagrus fulvidraco%antioxidant capacity%complement C3%complement C4
研究饲料中添加维生素D3对黄颡鱼（ Pelteobagrus fulvidraco）幼鱼血清和肝脏抗氧化功能以及血清补体C3、C4含量的影响。试验选取黄颡鱼（2.0±0.0） g 900尾，随机分为6组，每组设3缸重复，每缸50尾鱼。基础饲料以酪蛋白和明胶为蛋白源，糊精为糖源，豆油为脂肪源。在基础饲料中添加包膜饲料级维生素D3配制成维生素D3含量分别为0，324，1243，3621，8040和22700 IU/kg的6组饲料。试验持续12周。结果显示，与对照组相比，维生素D3添加组血清和肝脏超氧化物歧化酶（SOD）活性显著提高（P＜0.05），而肝脏脂质过氧化物（ LPO）含量显著降低（ P＜0.05），但维生素D3最高剂量添加组LPO含量升高。各维生素D3添加组肝脏过氧化氢酶（CAT）活性呈先升高后降低趋势（P＜0.05），其中维生素D3含量为3621 IU/kg组活性最高。血清CAT活性、 C3含量、 C4含量，血清和肝脏丙二醛（ MDA）含量不受饲料中维生素D3含量影响。结果表明，维生素D3含量为324～3621 IU/kg的饲料可提高黄颡鱼幼鱼抗氧化能力。
研究飼料中添加維生素D3對黃顙魚（ Pelteobagrus fulvidraco）幼魚血清和肝髒抗氧化功能以及血清補體C3、C4含量的影響。試驗選取黃顙魚（2.0±0.0） g 900尾，隨機分為6組，每組設3缸重複，每缸50尾魚。基礎飼料以酪蛋白和明膠為蛋白源，糊精為糖源，豆油為脂肪源。在基礎飼料中添加包膜飼料級維生素D3配製成維生素D3含量分彆為0，324，1243，3621，8040和22700 IU/kg的6組飼料。試驗持續12週。結果顯示，與對照組相比，維生素D3添加組血清和肝髒超氧化物歧化酶（SOD）活性顯著提高（P＜0.05），而肝髒脂質過氧化物（ LPO）含量顯著降低（ P＜0.05），但維生素D3最高劑量添加組LPO含量升高。各維生素D3添加組肝髒過氧化氫酶（CAT）活性呈先升高後降低趨勢（P＜0.05），其中維生素D3含量為3621 IU/kg組活性最高。血清CAT活性、 C3含量、 C4含量，血清和肝髒丙二醛（ MDA）含量不受飼料中維生素D3含量影響。結果錶明，維生素D3含量為324～3621 IU/kg的飼料可提高黃顙魚幼魚抗氧化能力。
연구사료중첨가유생소D3대황상어（ Pelteobagrus fulvidraco）유어혈청화간장항양화공능이급혈청보체C3、C4함량적영향。시험선취황상어（2.0±0.0） g 900미，수궤분위6조，매조설3항중복，매항50미어。기출사료이락단백화명효위단백원，호정위당원，두유위지방원。재기출사료중첨가포막사료급유생소D3배제성유생소D3함량분별위0，324，1243，3621，8040화22700 IU/kg적6조사료。시험지속12주。결과현시，여대조조상비，유생소D3첨가조혈청화간장초양화물기화매（SOD）활성현저제고（P＜0.05），이간장지질과양화물（ LPO）함량현저강저（ P＜0.05），단유생소D3최고제량첨가조LPO함량승고。각유생소D3첨가조간장과양화경매（CAT）활성정선승고후강저추세（P＜0.05），기중유생소D3함량위3621 IU/kg조활성최고。혈청CAT활성、 C3함량、 C4함량，혈청화간장병이철（ MDA）함량불수사료중유생소D3함량영향。결과표명，유생소D3함량위324～3621 IU/kg적사료가제고황상어유어항양화능력。
This experiment was conducted to investigate the effects of dietary vitamin D 3 supplementation on the serum and liver antioxidant capacities and serum complement C 3 and complement C4 contents in juvenile Pelteobagrus fulvidraco.For the basal diet , casein and gelatin were used as protein sources , dextrin as the carbohydrate source , and soybean oil as the lipid source.Six experimental diets were formulated to contain graded levels of vitamin D 3 (0, 324, 124 3, 362 1, 804 0 and 227 00 IU/kg, respectively).Each diet was assigned to triplicate tanks of 50 juvenile P.fulvidraco (2.0 ±0.0 g) for 12 weeks.The results were as follows: vitamin D3 supplementation significantly improved activities of superoxide dis-mutase ( SOD) in serum and liver ( P<0.05 ) , accompanying with decrease of liver lipid hydroperoxide ( LPO) level ( P<0.05 ) , however , fish fed the highest amount of vitamin D 3 tended to have higher liver LPO level .Among the vitamin D3 addition groups , liver catalase ( CAT ) activity increased from 324 IU/kg group to 362 1 IU/kg group and then de-creased (P<0.05).Dietary vitamin D3 had no effect on serum CAT activities and serum complement C 3, serum comple-ment C4, serum MDA and liver MDA contents .In conclusion, vitamin D3 addition improved antioxidant capacity and health condition of juvenile P.fulvidraco, and the recommended vitamin D 3 level in the diet was 324~362 1 IU/kg.
