CAJ | 학술논문

目的：建立西吡氯铵滴眼液中吡啶的气相色谱测定法。方法：用5%二苯基-95%二甲基硅氧烷共聚物为固定相的毛细管柱（30m×0.54mm×5.0μm），柱温在35℃维持5分钟，然后以每分钟8℃的升温速率升至115℃，以氮气为载气，气化室和检测器温度分别为120℃和260℃。结果：吡啶与杂质峰之间的分离良好。在0.492~9.84μg/ml的范围内吡啶的峰面积和浓度线性相关，回收率99.8%（n=9），RSD为4.9%（n=6）。结论：该分析方法准确可靠，简便易行，可用于西吡氯铵滴眼液中吡啶的测定。
목적：건립서필록안적안액중필정적기상색보측정법。방법：용5%이분기-95%이갑기규양완공취물위고정상적모세관주（30m×0.54mm×5.0μm），주온재35℃유지5분종，연후이매분종8℃적승온속솔승지115℃，이담기위재기，기화실화검측기온도분별위120℃화260℃。결과：필정여잡질봉지간적분리량호。재0.492~9.84μg/ml적범위내필정적봉면적화농도선성상관，회수솔99.8%（n=9），RSD위4.9%（n=6）。결론：해분석방법준학가고，간편역행，가용우서필록안적안액중필정적측정。
Objective: To establish a gas chromatography method for the determination of pyridine in cetylpyridinium chloride ophthalmic solution. Methods: The separation was performed on a capillary column which was a copolymer of 5% diphenyl-95% diphenyl siloxane as stationary phase.Column temperature was 35℃for 5 minutes.Then it increased to 115℃in rate of 8℃per minute.Carrier gas was nitrogen. Injector and detector were set at 120℃and 260℃respectively. Results:Pyridine and contamination could be completely separated with the established method. It was a linear correlation between the peak area and the concentration in concentration range of 0.492~9.84μg/ml.The recovery was 99.8%(n=9).RSD is 4.9%(n=6). Conclusion: The established method was accurate, simple and is suitable for determination of pyridine in cetylpyridinium chloride ophthalmic solution.