检验检疫学刊
檢驗檢疫學刊
검험검역학간
INSPECTION AND QUARANTINE SCIENCE
2013年
3期
42-45
,共4页
龙再浩%孙运%马中春%李冰
龍再浩%孫運%馬中春%李冰
룡재호%손운%마중춘%리빙
β-乳球蛋白%荧光偏振免疫分析%婴幼儿配方奶粉
β-乳毬蛋白%熒光偏振免疫分析%嬰幼兒配方奶粉
β-유구단백%형광편진면역분석%영유인배방내분
β-Lactoglobulin%Fluorescence Polarization Immunoassay%Infant Formula
使用荧光偏振免疫分析技术分析婴幼儿奶粉中牛乳蛋白β-乳球蛋白水平。通过免疫家兔制备抗β-乳球蛋白的多克隆抗体，合成异硫氰酸荧光素I标记的示踪物，在优化条件下，建立β-乳球蛋白的荧光偏振免疫技术检测方法。方法检测线性范围为0.22mg/L-2.53mg/L，IC50为0.75mg/L，检出限为0.09mg/L。本方法操作简便、快速，适合筛查婴幼儿配方奶粉中β-乳球蛋白。
使用熒光偏振免疫分析技術分析嬰幼兒奶粉中牛乳蛋白β-乳毬蛋白水平。通過免疫傢兔製備抗β-乳毬蛋白的多剋隆抗體，閤成異硫氰痠熒光素I標記的示蹤物，在優化條件下，建立β-乳毬蛋白的熒光偏振免疫技術檢測方法。方法檢測線性範圍為0.22mg/L-2.53mg/L，IC50為0.75mg/L，檢齣限為0.09mg/L。本方法操作簡便、快速，適閤篩查嬰幼兒配方奶粉中β-乳毬蛋白。
사용형광편진면역분석기술분석영유인내분중우유단백β-유구단백수평。통과면역가토제비항β-유구단백적다극륭항체，합성이류청산형광소I표기적시종물，재우화조건하，건립β-유구단백적형광편진면역기술검측방법。방법검측선성범위위0.22mg/L-2.53mg/L，IC50위0.75mg/L，검출한위0.09mg/L。본방법조작간편、쾌속，괄합사사영유인배방내분중β-유구단백。
A fluorescence polarization immunoassay (FPIA) method was developed for detecting theβ-lactoglobulin in infant formula. Rabbits were immunized to produce polyclonal antibody forβ-lactoglobulin. Tracer was synthesized with FITC fluorescence. On optimal conditions, a calibration curve showed a good linearity in the range of 0.22mg/L-2.53mg/L. The detection limit was 0.09mg/L and IC50 was 0.75mg/L. The method was simple, rapid and suitable for screening the level ofβ-lactoglobulin in infant formula.
