CAJ | 학술논문

目的了解毛囊干细胞在常见瘢痕性脱发和非瘢痕性脱发中的变化及意义。方法采用SP免疫组化法检测毛囊干细胞常用标志分子CK15、CD200在常见瘢痕性脱发和非瘢痕性脱发即盘状红斑狼疮（DLE）和斑秃皮损中的表达。每组8例患者，以同等数量正常人头皮做对照。结果毛囊干细胞标志CK15、CD200在正常对照的阳性率均为100%，而在DLE患者头皮中的阳性率分别为37.5%和12.5%，同正常对照相比均明显减少（P＜0.05）；斑秃患者毛囊中CK15阳性表达率为100%，CD200阳性表达率为50%，CD200阳性表达率与正常对照相比明显减少（P＜0.05）。结论毛囊干细胞在DLE患者中明显受损，可能与其永久性脱发有关；而在斑秃中毛囊干细胞无减少。CD200在毛囊表达减少可能与斑秃及DLE的发病相关。
목적료해모낭간세포재상견반흔성탈발화비반흔성탈발중적변화급의의。방법채용SP면역조화법검측모낭간세포상용표지분자CK15、CD200재상견반흔성탈발화비반흔성탈발즉반상홍반랑창（DLE）화반독피손중적표체。매조8례환자，이동등수량정상인두피주대조。결과모낭간세포표지CK15、CD200재정상대조적양성솔균위100%，이재DLE환자두피중적양성솔분별위37.5%화12.5%，동정상대조상비균명현감소（P＜0.05）；반독환자모낭중CK15양성표체솔위100%，CD200양성표체솔위50%，CD200양성표체솔여정상대조상비명현감소（P＜0.05）。결론모낭간세포재DLE환자중명현수손，가능여기영구성탈발유관；이재반독중모낭간세포무감소。CD200재모낭표체감소가능여반독급DLE적발병상관。
Objective To investigate the changes of hair follicle stem cells and their signiifcance in the common scaring alopecia and non-scaring alopecia caused by discoid lupus erythematosus (DLE) and alopecia areata (AA). Methods Immunohistochemical method was performed to detect CK15 (cytokeratin 15) and CD200, two markers of hair follicle stem cells, in the alopecia lesions of 16 patients with DLE(8 cases) or AA(8 cases). Samples from healthy people after plastic surgery were as the controls. Results The positive expression of CK15 and CD200 in the follicles of DLE lesions was 37.5%and 12.5%respectively, signiifcantly diminished compared with that in healthy controls (both are 100%, P<0.05). The positive expression of CK15 in follicles of AA lesions was normal, while the positive expression of CD200 was 50%, signiifcantly diminished compared with that in healthy controls (P<0.05). Conclusion Hair follicle stem cells were signiifcantly damaged in scarring alopecia caused by DLE but not AA, meanwhile the expression of CD200 decreased in follicles of patients with AA. The damage of hair follicle stem cells may be associated with the irreversible loss of hair follicles in DLE, and the low expression of CD200 may be associated with the pathogenesis of DLE and AA.