CAJ | 학술논문

目的：探讨表皮生长因子(epidermal growth factor，EGF)和碱性成纤维生长因子（basic fibroblast growth factor， bFGF）对人牙髓干细胞(human dental pulp stem cells， hDPSCs)增殖能力的影响。方法：采用噻唑蓝（MTT）比色法分别测定不同浓度EGF和bFGF在不同时间，单独或联合应用对hDPSCs增殖能力的影响。结果：50 ng/ml EGF和20 ng/ml bFGF促hDPSCs增殖能力最强。以50ng/ml EGF、20ng/ml bFGF单独或联合作用，其促hDPSCs增殖能力均具有时间依赖性，第3 d、5 d、7 d， EGF、bFGF促hDPSCs增殖作用显著增强，二种生长因子联合作用，促增殖作用显著高于其单独作用效果，差异具有统计学意义（P<0.05）。结论：生长因子EGF和bFGF最大效应浓度分别为：50 ng/ml、20 ng/ml。EGF和bFGF单独作用均能显著促进hDPSCs的增殖，二者联合作用对hDPSCs的增殖有一定的协同作用。
목적：탐토표피생장인자(epidermal growth factor，EGF)화감성성섬유생장인자（basic fibroblast growth factor， bFGF）대인아수간세포(human dental pulp stem cells， hDPSCs)증식능력적영향。방법：채용새서람（MTT）비색법분별측정불동농도EGF화bFGF재불동시간，단독혹연합응용대hDPSCs증식능력적영향。결과：50 ng/ml EGF화20 ng/ml bFGF촉hDPSCs증식능력최강。이50ng/ml EGF、20ng/ml bFGF단독혹연합작용，기촉hDPSCs증식능력균구유시간의뢰성，제3 d、5 d、7 d， EGF、bFGF촉hDPSCs증식작용현저증강，이충생장인자연합작용，촉증식작용현저고우기단독작용효과，차이구유통계학의의（P<0.05）。결론：생장인자EGF화bFGF최대효응농도분별위：50 ng/ml、20 ng/ml。EGF화bFGF단독작용균능현저촉진hDPSCs적증식，이자연합작용대hDPSCs적증식유일정적협동작용。
Objective: To investigate the effects of epidermal growth factor (EGF) and basic fibroblast growth factor(bFGF)on the proliferation of human dental pulp stem cells(hDPSCs) in vitro. Methods: Colorimetric assay was used to measure the dose - and time - depedent effects of EGF and bFGF on the proliferation of hDPSCs. Results:50 ng/ml EGF and 20 ng/ml bFGF had the strongeste effect on promoting the proliferation of hDPSCs re-spectively. The effect of 50 ng/ml EGF and 20 ng/ml bFGF, whether alone or combined was time-depedent. It was significantly stronger at 3, 5, 7 d (P < 0.05), and the combined usege of the two cytokines was obviously stronger than used alone(P<0.05).Conclusion: The optimal concentration of EGF and bFGF is 50 ng/ml and 20 ng/ml respectively. Both EGF and bFGF can significantly promote the proliferation of hDPSCs. The combination use of two growth factors has a certain synergism.