CAJ | 학술논문

目的：大鼠环孢霉素A中毒性肾损伤模型的探讨。方法：实验大鼠随机分为对照组，不同浓度的环孢霉素A给药组，分别给予橄榄油灌胃，不同浓度的环孢霉素A灌胃，连续4w后，测动物体重，24 h尿量，尿蛋白，取血测血肌酐、尿素氮，取肾组织作病理观察并行肾小管损伤评分，测组织超氧化物歧化酶和丙二醛含量。结果：与对照组比，25 mg/kg/d环孢霉素灌胃组肾组织结构变化明显，但功能改变不明显，50 mg/kg/d环孢霉素灌胃组肾脏形态结构及功能受损均明显，尿蛋白含量增多，血肌酐、尿素氮明显增高（P<0.05）；肾组织超氧化物歧化酶含量减低而氧化产物丙二醛MDA含量明显增高（P<0.05）。结论：持续4周50 mg/kg/d剂量环孢霉素A灌胃可复制典型的环孢霉素中毒性肾损伤模型。
목적：대서배포매소A중독성신손상모형적탐토。방법：실험대서수궤분위대조조，불동농도적배포매소A급약조，분별급여감람유관위，불동농도적배포매소A관위，련속4w후，측동물체중，24 h뇨량，뇨단백，취혈측혈기항、뇨소담，취신조직작병리관찰병행신소관손상평분，측조직초양화물기화매화병이철함량。결과：여대조조비，25 mg/kg/d배포매소관위조신조직결구변화명현，단공능개변불명현，50 mg/kg/d배포매소관위조신장형태결구급공능수손균명현，뇨단백함량증다，혈기항、뇨소담명현증고（P<0.05）；신조직초양화물기화매함량감저이양화산물병이철MDA함량명현증고（P<0.05）。결론：지속4주50 mg/kg/d제량배포매소A관위가복제전형적배포매소중독성신손상모형。
Objective: To explore the establishment of nephrotoxicity model by Cyclosporin A(CsA).Methods:Rats in 3 groups were treated with olive oil (control) and CsA of two different doses respectively.After 4 weeks , nephrotoxicity was assessed by the examination of body weight, serum creatinine, blood urea nitrogen, activity of antioxitant and histopathological changes. The injury of renal tubules was scored. Results: Both histopathological and functional damages were significant in CsA 50mg/kg/d group; the functional change was less conspicuous in CsA 25mg/kg/d group. Conclusion: The result indicates that a nephrotoxicity animal model can be replicated by 4 weeks intragastric administration of cyclosporine at the concentration of 50 mg/kg/d.