CAJ | 학술논문

目的：建立卵母细胞双电极电压钳记录技术。方法：建立双电极电压钳技术平台，并应用此平台，在去除滤泡膜的中国四川牛蛙卵母细胞上，记录内源性的外向离子电流。结果：卵母细胞孵育在正常ND96溶液中，将细胞膜钳制在-80 mV膜电位，给予一系列的去极化刺激可诱发出一个外向电流。在细胞外液中加入10 mM的TEA可阻断该电流80%以上，洗脱后电流幅值恢复至90%左右。将正常ND96溶液替换为无钙ND96溶液后，电流没有变化。结论：成功建立了卵母细胞双电极电压钳记录技术。去极化刺激在牛蛙卵母细胞上诱发出的外向电流为钾电流。
목적：건립란모세포쌍전겁전압겸기록기술。방법：건립쌍전겁전압겸기술평태，병응용차평태，재거제려포막적중국사천우와란모세포상，기록내원성적외향리자전류。결과：란모세포부육재정상ND96용액중，장세포막겸제재-80 mV막전위，급여일계렬적거겁화자격가유발출일개외향전류。재세포외액중가입10 mM적TEA가조단해전류80%이상，세탈후전류폭치회복지90%좌우。장정상ND96용액체환위무개ND96용액후，전류몰유변화。결론：성공건립료란모세포쌍전겁전압겸기록기술。거겁화자격재우와란모세포상유발출적외향전류위갑전류。
Objective: To establish two-microelectrode voltage-clamp technique for oocytes. Methods: Two-microelectrode voltage-clamp technique platform was established and the endogenous outward ionic currents were recorded on bullfrog oocytes after the removal of follicular theca. Results: As the oocytes were incubated in normal ND96 solution, the membrane potential was maintained at -80 mV, then depolarized to +50 mV in 10 mV increments, the outward cuttents were recorded. After applied 10 mmol/L TEA in the extracellular fluid, more than 80% currents were blocked, then recovery to about 90% after washout. The currents did not obviously change in ND96 solution without calcium. Conclusion: Two -microelectrode voltage -clamp technique is successfully established in oocytes. The outward currents induced by depolarizing stimulation on bullfrog oocytes were potassium currents.