CAJ | 학술논문

为筛选卵黄抗体的优良佐剂，以3株猪源致病性大肠杆菌（ETEC）菌毛蛋白为模式抗原，分别与6种佐剂制备多价菌毛蛋白疫苗，免疫海兰褐壳蛋鸡。结果显示，3次免疫后，所有试验组蛋鸡产蛋率均有不同程度下降，但 SPA、ISCOMs、弗氏佐剂、蜂胶佐剂组的产蛋率下降程度较为轻微；对鸡体血清抗体和制备获得的卵黄抗体的效价检测结果均显示：SPA和ISCOMs组虽较SPB、SPC、弗氏佐剂组抗体水平略低，但持久度较好，且通过对蛋鸡外观形态和注射部位组织变化情况的观察显示，两者不会刺激鸡体热反应或炎症反应，无任何表观副作用。因此，更适合作为蛋鸡制备猪源ETEC卵黄抗体的优良佐剂。
위사선란황항체적우량좌제，이3주저원치병성대장간균（ETEC）균모단백위모식항원，분별여6충좌제제비다개균모단백역묘，면역해란갈각단계。결과현시，3차면역후，소유시험조단계산단솔균유불동정도하강，단 SPA、ISCOMs、불씨좌제、봉효좌제조적산단솔하강정도교위경미；대계체혈청항체화제비획득적란황항체적효개검측결과균현시：SPA화ISCOMs조수교SPB、SPC、불씨좌제조항체수평략저，단지구도교호，차통과대단계외관형태화주사부위조직변화정황적관찰현시，량자불회자격계체열반응혹염증반응，무임하표관부작용。인차，경괄합작위단계제비저원ETEC란황항체적우량좌제。
immunologic adjuvants were mixed with ETEC(K88,K99,and 987P)model antigens and used to stimulate Hy-line Variety Brown hen to produce egg yolk antibodies. The results showed that the egg productions of Group SPA,ISCOMs,FA (Freund’s adju-vant),and Propolis dreased lower than the other groups. And Group SPA and ISCOMs did not show any heating reaction or inflamma-tion after the injection immunity although the tilers of serum and egg yolk antibodies of them were slightly lower than Group SPB,SPC and FA. Thus we came to a conclusion that Group SPA and ISCOMs were better to be developed as potential adjuvats for egg yolk an-tibodies production.